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 were able to identify hepatocytes in which caspases were activated. Segmental normothermic ischemia of the liver was induced for 120 minutes. Six hours post-reperfusion, FAM-FLIVO was injected into the portal veins of the rats and left to circulate for 10 minutes. Images were taken from 5 um cryosections of liver with the nuclei visualized in blue using DAPI (400X). A bright green signal is clearly seen in hepatocytes containing active caspases distributed around the vessel in the ischemic condition (right) compared to the control non-ischemic tissue (left).MBS258407_TD2.jpg "Testing Data (CASPASE ACTIVITY IN RAT BRAIN. Using FAM-FLIVO to monitor cell death, there is a clear distinction between healthy and apoptotic neurons. In this live animal brain study of diabetes, Dr. Thomas Morrow at the University of Michigan VAMC Ann Arbor was able to assess neurodegeneration via caspase activity in control (left) and 8-week STZ diabetic rats (right). 30 minutes prior to sacrifice, FAM-FLIVO was injected intravenously to directly label caspase-positive apoptotic neurons. After sacrifice, 20 µm frozen sections of the periaqueductal gray (PAG) were prepared and counter-stained with red fluorescent Nissl to identify all neurons. Dying apoptotic neurons exhibit dual staining with FAM-FLIVO (yellow/green) and Nissl (red). In this model, diabetic animals show greater levels of caspase activity in the PAG than control animals.)")
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Enhanced Antifade Mounting Medium with DAPI
(#MBS9719255)
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