Alamar Blue Staining Reagent – Biochem-Stains & Dyes

Molecular Biology Grade

May be stored at 4°C, -20°C or -70°C. When stored frozen, it is important to warm to 37°C upon thawing and mixed well to be assured of complete resolubilization. Protect from light. Storage at RT under lighted conditions adversely affects its absorbance properties.

The innate metabolic activity of cells can be monitored with Alamar Blue™. In the presence of added toxic compounds such as antimicrobial agents, this innate metabolic activity ceases. Procedures which cite the use of Alamar Blue™ in assays of efficacy of antimicrobial agents call for the determination of Alamar Blue™ reduction at a single predetermined time point.

Alamar BlueTM is soluble, stable in culture medium and is non-toxic. The continuous monitoring of cells in culture is therefore permitted. Specifically, Alamar Blue™ does not alter the viability of cells cultured for various times as monitored by Trypan Blue exclusion. Cells grown in the presence of Alamar Blue™ and subsequently analyzed by Flow Cytometry for CD44, CD45RB, CD4 and heat stable antigen are found to produce similar numbers of viable cells and antigen expressing cells as non- Alamar Blue™ exposed cells. Because Alamar Blue™ is non-toxic, the cells under study can be returned to culture or used for other purposes including histological studies. Proliferation measurements with Alamar Blue™ may be made either spectrophotometrically by monitoring the absorption of Alamar Blue™ supplemented cell culture media at two wavelengths. Alternatively, proliferation measurements with Alamar Blue™ may bemade fluorometrically .

Biochemicals; Biochem-Stains & Dyes