Dopachrome Tautomerase (DT) ELISA Kit for Human

Human

Sandwich

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

0.625 ng/ml - 20 ng/ml.

0.1 ng/ml.

No significant cross-reactivity or interference between Human DT and analogues was observed.

Store all reagents at 2-8 degree C

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay CV (%) is less than 15%.

59,145 Da

Chromosome: 13; NC_000013.10 (95091835..95131936, complement). Location: 13q32

191275

ModBase 3D Structure for P40126

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated DT concentrations in Human serum, plasma and other body fluids. Using Purified Human DT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DT and DT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DT in the samples is then determined by comparing the O.D. of the samples to the standard curve.