Defensin 1, Neutrophil (DEFA1) ELISA Kit for Human

Name: Human DEFA1 ELISA Kit; Defensin 1, Neutrophil; MyBioSource
Brand: MyBioSource
SKU: MBS016501
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS016501

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Defensin 1, Neutrophil (DEFA1), ELISA Kit

Full Product Name

Human Defensin 1, Neutrophil ELISA Kit

Product Gene Name

DEFA1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

31.2 pg/ml - 1000 pg/ml.

Sensitivity

5.0 pg/ml.

Specificity

No significant cross-reactivity or interference between Human DEFA1 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

10,201 Da

OMIM

125220

3D Structure

ModBase 3D Structure for P59665

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of DEFA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEFA1 purchase

MBS016501 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Defensin 1, Neutrophil (DEFA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DEFA1. The ELISA analytical biochemical technique of the MBS016501 kit is based on DEFA1 antibody-DEFA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DEFA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DEFA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DEFA1. It may not necessarily be applicable to this product.

NCBI GI #

85567619

NCBI GeneID

1667

NCBI Accession #

AAI12189.1

UniProt Primary Accession #

P59665

UniProt Secondary Accession #

P11479; Q14125; Q6EZF6

UniProt Related Accession #

P59665

NCBI Official Full Name

Defensin, alpha 1, preproprotein

NCBI Official Synonym Full Names

defensin, alpha 1

NCBI Official Symbol

DEFA1

NCBI Official Synonym Symbols

HP1; MRS; DEF1; HP-1; DEFA2; HNP-1

UniProt Protein Name

Neutrophil defensin 1

UniProt Synonym Protein Names

Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2

Protein Family

Alpha-defensin

UniProt Gene Name

DEFA1

UniProt Synonym Gene Names

DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

UniProt Entry Name

DEF1_HUMAN

NCBI Protein Information

neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence

NCBI Summary for DEFA1

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

UniProt Comments for DEFA1

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus

Research Articles on DEFA1

1. A decrease in salivary HNP 1-3 levels might be a biological factor for predisposition to oral ulcers in patients with Behcet disease and oral infection in healthy patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DEFA1 elisa kit

Reference Product	PubMed Publications
DEFA3 elisa kit	>11 publications with DEFA1 and DEFA3
DEFB1 elisa kit	>5 publications with DEFA1 and DEFB1
DEFB4A elisa kit	>1 publications with DEFA1 and DEFB4A

Pathways associated with DEFA1 elisa kit

Products by Pathway	Pathway Diagram
Alpha-defensins Pathway antibodies	Alpha-defensins Pathway Diagram
Cellular Roles Of Anthrax Toxin Pathway antibodies	Cellular Roles Of Anthrax Toxin Pathway Diagram
Defensins Pathway antibodies	Defensins Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with DEFA1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>9 publications with DEFA1 and Inflammation
Neoplasms Antibodies	>3 publications with DEFA1 and Neoplasms
Liver Diseases Antibodies	>1 publications with DEFA1 and Liver Diseases
Adenocarcinoma Antibodies	>1 publications with DEFA1 and Adenocarcinoma
Craniofacial Abnormalities Antibodies	>1 publications with DEFA1 and Craniofacial Abnormalities
Leukemia Antibodies	>1 publications with DEFA1 and Leukemia
Nervous System Diseases Antibodies	>1 publications with DEFA1 and Nervous System Diseases
Fibrosis Antibodies	>1 publications with DEFA1 and Fibrosis
Necrosis Antibodies	>1 publications with DEFA1 and Necrosis
Neoplasm Metastasis Antibodies	>1 publications with DEFA1 and Neoplasm Metastasis

Organs/Tissues associated with DEFA1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>18 publications with DEFA1 and Blood
Bone Antibodies	>3 publications with DEFA1 and Bone
Liver Antibodies	>2 publications with DEFA1 and Liver
Bone Marrow Antibodies	>1 publications with DEFA1 and Bone Marrow