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Replication Factor C Subunit 4 (RFC4) Saccharomyces Cerevisiae Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1103088

MBS1103088 is a recombinant protein targeting RFC4. Also known as Recombinant Saccharomyces cerevisiae Replication factor C subunit 4 (RFC4). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1035 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Replication factor C subunit 4 (RFC4), Recombinant Protein
Full Product Name
Recombinant Saccharomyces cerevisiae Replication factor C subunit 4 (RFC4)
Product Gene Name
RFC4 recombinant protein
Sequence
MSKTLSLQLP WVEKYRPQVL SDIVGNKETI DRLQQIAKDG NMPHMIISGM PGIGKTTSVH CLAHELLGRS YADGVLELNA SDDRGIDVVR NQIKHFAQKK LHLPPGKHKI VILDEADSMT AGAQQALRRT MELYSNSTRF AFACNQSNKI IEPLQSRCAI LRYSKLSDED VLKRLLQIIK LEDVKYTNDG LEAIIFTAEG DMRQAINNLQ STVAGHGLVN ADNVFKIVDS PHPLIVKKML LASNLEDSIQ ILRTDLWKKG YSSIDIVTTS FRVTKNLAQV KESVRLEMIK EIGLTHMRIL EGVGTYLQLA SMLAKIHKLN NKA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
36,149 Da
Sequence Positions
1-323, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P40339
Species
Saccharomyces cerevisiae (strain 204508 / S288c) (Baker's yeast)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of RFC4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RFC4. It may not necessarily be applicable to this product.

NCBI GI #
6324478
NCBI GeneID
854059
NCBI Accession #
NP_014547.1
UniProt Primary Accession #
P40339
UniProt Secondary Accession #
D6W1X4
UniProt Related Accession #
P40339
GenBank Nucleotide #
NM_001183348.1
NCBI Official Full Name
replication factor C subunit 4
NCBI Official Symbol
RFC4
UniProt Protein Name
Replication factor C subunit 4
UniProt Synonym Protein Names
Activator 1 37 kDa subunit
Protein Family
Probable replication factor
UniProt Gene Name
RFC4
UniProt Synonym Gene Names
Replication factor C4
NCBI Protein Information
replication factor C subunit 4

UniProt Comments for RFC4

Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RFC4 recombinant protein

 Reference Product  PubMed Publications
 RFC3 recombinant protein  >27 publications with RFC4 and RFC3
 RFC2 recombinant protein  >26 publications with RFC4 and RFC2
 RFC5 recombinant protein  >18 publications with RFC4 and RFC5
 RFC1 recombinant protein  >9 publications with RFC4 and RFC1
 RAD24 recombinant protein  >6 publications with RFC4 and RAD24
 CTF18 recombinant protein  >3 publications with RFC4 and CTF18
 RFA1 recombinant protein  >1 publications with RFC4 and RFA1
 ELG1 recombinant protein  >1 publications with RFC4 and ELG1
 POL30 recombinant protein  >1 publications with RFC4 and POL30

Pathways associated with RFC4 recombinant protein

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Strand Elongation Pathway antibodies  DNA Strand Elongation Pathway Diagram
 Extension Of Telomeres Pathway antibodies  Extension Of Telomeres Pathway Diagram
 Lagging Strand Synthesis Pathway antibodies  Lagging Strand Synthesis Pathway Diagram
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