• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GTP Pyrophosphokinase Ywac (ywaC) Bacillus Subtilis Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1121732

MBS1121732 is a recombinant protein targeting ywaC. Also known as Recombinant Bacillus subtilis GTP pyrophosphokinase ywaC (ywaC). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$865 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GTP pyrophosphokinase ywaC (ywaC), Recombinant Protein
Full Product Name
Recombinant Bacillus subtilis GTP pyrophosphokinase ywaC (ywaC)
Product Gene Name
ywaC recombinant protein
Sequence
MDLSVTHMDD LKTVMEDWKN ELLVYKFALD ALDTKFSIIS QEYNLIHGHN PIEHTKSRVK SFESIVNKLM RKGCEITTKE MKEHIHDIAG VRIICSFISD IYNVVNVLKQ HEDLRIVKVK DYIQTPKPNG YRSLHLIIEM PVNLTNRVEY VKAEIQIRTI AMDFWASLEH KIYYKLNNDV PKQLTDELKE AAEIAHYLDE KMLGIKKEVD
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
24,600 Da
Sequence Positions
1-210, Full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P39583
Species
Bacillus subtilis (strain 168)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ywaC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ywaC. It may not necessarily be applicable to this product.

NCBI GI #
16080899
NCBI GeneID
937355
NCBI Accession #
NP_391727.1
UniProt Primary Accession #
P39583
UniProt Related Accession #
P39583
GenBank Nucleotide #
NC_000964.3
NCBI Official Full Name
GTP pyrophosphokinase YwaC
NCBI Official Symbol
ywaC
UniProt Protein Name
GTP pyrophosphokinase YwaC
UniProt Synonym Protein Names
(p)ppGpp synthase YwaC; Small alarmone synthase 2
Protein Family
GTP pyrophosphokinase
UniProt Gene Name
ywaC
UniProt Synonym Gene Names
SAS 2
NCBI Protein Information
GTP pyrophosphokinase YwaC

UniProt Comments for ywaC

Functions as a (p)ppGpp synthase; GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis (PubMed:18067544). Overexpression in relA mutants (triple relA-yjbM-ywaC deletions and single relA deletions) leads to growth arrest; GTP levels fall drastically, various guanine-related nucleotides are synthesized (ppGp or pGpp), the cellular transcriptional profile changes dramatically and 70S ribosome dimerization occurs (PubMed:22950019). Overexpression in the presence of a wild-type relA gene does not have these effects (PubMed:22950019). In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response (PubMed:18067544).
Show more

Research Articles on ywaC

1. The authors found that transcription of all rrn operons from their P1 promoters was drastically reduced in the relA mutant while this was almost completely restored in the yjbM and ywaC suppressor mutants.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ywaC recombinant protein

 Reference Product  PubMed Publications
 yjcH recombinant protein  >1 publications with ywaC and yjcH
 yfhM recombinant protein  >1 publications with ywaC and yfhM

Pathways associated with ywaC recombinant protein

 Products by Pathway  Pathway Diagram
 PpGpp Biosynthesis Pathway antibodies  PpGpp Biosynthesis Pathway Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000