Terminal Complement Complex C5b-9, TCC C5b-9 (TCC C5B-9) ELISA Kit for Rat

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TCC C5b-9 antibody. TCC C5b-9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TCC C5b-9 Antibody is added and binds to TCC C5b-9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TCC C5b-9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TCC C5b-9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat terminal complement complex C5b-9 (also known as TCC C5b-9) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Small volumes of TCC C5b-9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS161908 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the terminal complement complex C5b-9, TCC C5b-9, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TCC C5b-9. The ELISA analytical biochemical technique of the MBS161908 kit is based on TCC C5b-9 antibody-TCC C5b-9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TCC C5b-9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TCC C5b-9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).