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Blue Light- and Temperature-Regulated Antirepressor Ycgf (ycgF) Escherichia Coli Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1152429

MBS1152429 is a recombinant protein targeting ycgF. Also known as Recombinant Escherichia coli Blue light- and temperature-regulated antirepressor YcgF (ycgF). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1140 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Blue light- and temperature-regulated antirepressor YcgF (ycgF), Recombinant Protein
Full Product Name
Recombinant Escherichia coli Blue light- and temperature-regulated antirepressor YcgF (ycgF)
Product Gene Name
Sequence
MLTTLIYRSH IRDDEPVKKI EEMVSIANRR NMQSDVTGIL LFNGSHFFQL LEGPEEQVKM IYRAICQDPR HYNIVELLCD YAPARRFGKA GMELFDLRLH ERDDVLQAVF DKGTSKFQLT YDDRALQFFR TFVLATEQST YFEIPAEDSW LFIADGSDKE LDSCALSPTI NDHFAFHPIV DPLSRRIIAF EAIVQKNEDS PSAIAVGQRK DGEIYTADLK SKALAFTMAH ALELGDKMIS INLLPMTLVN EPDAVSFLLN EIKANALVPE QIIVEFTESE VISRFDEFAE AIKSLKAAGI SVAIDHFGAG FAGLLLLSRF QPDRIKISQE LITNVHKSGP RQAIIQAIIK CCTSLEIQVS AMGVATPEEW MWLESAGIEM FQGDLFAKAK LNGIPSIAWP EKK
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
45,295 Da
Sequence Positions
1-403, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P75990
Species
Escherichia coli (strain K12)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ycgF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ycgF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
anti-repressor for YcgE, blue light-responsive; FAD-binding; inactive c-di-GMP phosphodiesterase-like EAL domain protein
NCBI Official Symbol
bluF
NCBI Official Synonym Symbols
ECK1150; JW1150; ycgF
UniProt Protein Name
Blue light- and temperature-regulated antirepressor BluF
UniProt Gene Name
bluF
UniProt Synonym Gene Names
Blrp
NCBI Protein Information
anti-repressor for YcgE, blue light-responsive; FAD-binding; inactive c-di-GMP phosphodiesterase-like EAL domain protein

NCBI Summary for ycgF

YcgF has an N-terminal blue light-responsive FAD-binding BLUF domain. [More information is available at EcoGene: EG13887]. YcgF is a member of the family of blue-light sensing proteins that use FAD (BLUF). [More information is available at EcoCyc: G6603].

UniProt Comments for ycgF

Binds to and releases the BluR repressor from its bound DNA target in a blue light-dependent (470 nm) fashion. A shift to low temperature also triggers a BluF-mediated relief of repression by BluR, suggesting BluF may serve as a thermometer. Blue light may act to increase the affinity of BluF for BluR, allowing it to be released from its operator. The protein has a reversible photocycle, and undergoes structural changes, probably in the EAL domain, in response to light.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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