Apolipoprotein A4 (APOA4) ELISA Kit for Canine

Name: Canine APOA4 ELISA Kit; Apolipoprotein A4; MyBioSource
Brand: MyBioSource
SKU: MBS011841
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS011841

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of APOA4 (hereafter termed "analyte") in undiluted… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Apolipoprotein A4 (APOA4), ELISA Kit

Full Product Name

Canine Apolipoprotein A4 ELISA Kit

Product Gene Name

APOA4 elisa kit

Species Reactivity

Canine

Assay Type

Quantitative Sandwich

Detection Range

3.12ug/ml-100ug/ml

Sensitivity

1.0ug/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

45,399 Da

Chromosome Location

Chromosome: 11; NC_000011.9 (116691418..116694011, complement). Location: 11q23

OMIM

107690

3D Structure

ModBase 3D Structure for P06727

Other Notes

Small volumes of APOA4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOA4 purchase

MBS011841 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein A4 (APOA4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOA4. The ELISA analytical biochemical technique of the MBS011841 kit is based on APOA4 antibody-APOA4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOA4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOA4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for APOA4. It may not necessarily be applicable to this product.

NCBI GI #

71773110

NCBI GeneID

337

NCBI Accession #

NP_000473.2

UniProt Primary Accession #

P06727

UniProt Secondary Accession #

Q14CW8; Q6Q787; A8MSL6

UniProt Related Accession #

P06727

GenBank Nucleotide #

NM_000482.3

NCBI Official Full Name

apolipoprotein A-IV

NCBI Official Synonym Full Names

apolipoprotein A-IV

NCBI Official Symbol

APOA4

UniProt Protein Name

Apolipoprotein A-IV

UniProt Synonym Protein Names

Apolipoprotein A4

Protein Family

Apolipoprotein

UniProt Gene Name

APOA4

UniProt Synonym Gene Names

Apo-AIV; ApoA-IV

UniProt Entry Name

APOA4_HUMAN

NCBI Protein Information

apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4

NCBI Summary for APOA4

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

UniProt Comments for APOA4

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Lipid-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: antioxidant activity; lipid transporter activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; phototransduction, visible light; cholesterol metabolic process; multicellular organismal lipid catabolic process; removal of superoxide radicals; neurite regeneration; lipid homeostasis; protein-lipid complex assembly; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; cholesterol homeostasis; leukocyte adhesion; reverse cholesterol transport; regulation of cholesterol absorption; phospholipid efflux; innate immune response in mucosa; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport

Research Articles on APOA4

1. apoA-IV was increased in follicular fluid from polycystic ovary syndrome patients compared with those from normal controls

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with APOA4 elisa kit

Reference Product	PubMed Publications
APOC3 elisa kit	>37 publications with APOA4 and APOC3
APOA1 elisa kit	>31 publications with APOA4 and APOA1
LPL elisa kit	>14 publications with APOA4 and LPL
APOB elisa kit	>10 publications with APOA4 and APOB
APOC2 elisa kit	>6 publications with APOA4 and APOC2
APOA2 elisa kit	>4 publications with APOA4 and APOA2
TTR elisa kit	>1 publications with APOA4 and TTR
MTTP elisa kit	>1 publications with APOA4 and MTTP

Pathways associated with APOA4 elisa kit

Products by Pathway	Pathway Diagram
Amyloids Pathway antibodies	Amyloids Pathway Diagram
Chylomicron-mediated Lipid Transport Pathway antibodies	Chylomicron-mediated Lipid Transport Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Lipid Digestion, Mobilization, And Transport Pathway antibodies	Lipid Digestion, Mobilization, And Transport Pathway Diagram
Lipoprotein Metabolism Pathway antibodies	Lipoprotein Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram

Organs/Tissues associated with APOA4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>69 publications with APOA4 and Blood
Liver Antibodies	>16 publications with APOA4 and Liver
Intestine Antibodies	>4 publications with APOA4 and Intestine
Stomach Antibodies	>1 publications with APOA4 and Stomach