Intermediate Capsid Protein VP6 (VP6) Rotavirus A Recombinant Protein

Intermediate capsid protein that self assbles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid rain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP.

Cloning of bovine rotavirus (RF strain) nucleotide sequence of the gene coding for the major capsid protein.Cohen J., Lefevre F., Estes M.K., Bremont M.Virology 138:178-182(1984) Cohen J.Purification and characterization of bovine rotavirus cores.Bican P., Cohen J., Charpilienne A., Scherrer R.J. Virol. 43:1113-1117(1982) Identification of rotavirus VP6 residues located at the interface with VP2 that are essential for capsid assembly and transcriptase activity.Charpilienne A., Lepault J., Rey F.A., Cohen J.J. Virol. 76:7822-7831(2002) A zinc ion controls assembly and stability of the major capsid protein of rotavirus.Erk I., Huet J.-C., Duarte M., Duquerroy S., Rey F.A., Cohen J., Lepault J.J. Virol. 77:3595-3601(2003) Atomic structure of the major capsid protein of rotavirus implications for the architecture of the virion.Mathieu M., Petitpas I., Navaza J., Lepault J., Kohli E., Pothier P., Prasad B.V.V., Cohen J., Rey F.A.EMBO J. 20:1485-1497(2001)

Small volumes of VP6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1191284 is a recombinant protein targeting VP6. Also known as Recombinant Rotavirus A Intermediate capsid protein VP6. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.