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BTAF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120002

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of… More details

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Product Overview

Online Datasheet in PDF
Product Name
BTAF1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human BTAF1
Product Gene Name
anti-BTAF1 antibody
Product Synonym Names
BTAF1 RNA polymerase II, B-TFIID transcriptionfactor-associated, 170kDa (Mot1 homolog, S. cerevisiae) (BTAF1); MOT1; TAF172; KIAA0940; TAFII170;TAF(II)170
Clonality
Monoclonal
Isotype
IgG1
Clone Number
BTA3D61
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
76,419 Da
OMIM
605191
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-BTAF1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-BTAF1 antibody

Western blot analysis of immunized recombinant protein, using anti-BTAF1 monoclonal antibody.
Mouse Anti-BTAF1 Monoclonal Antibody – Cat# MBS120002

Quality Control #2 of anti-BTAF1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-BTAF1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120002

Western Blot (WB) of anti-BTAF1 antibody

Detection of BTAF1 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. BTA3D61-1]

Predicted molecular weight: 206 kDa
Mouse Anti-BTAF1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120002

Immunoprecipitation (IP) of anti-BTAF1 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BTAF1 mAb. [Lot No. BTA3D61-1]

Predicted molecular weight: 206 kDa
Mouse Anti-BTAF1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120002

Flow Cytometry (FC/FACS) of anti-BTAF1 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTAF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. BTA3D61-1]
Mouse Anti-BTAF1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120002

Immunocytochemistry (ICC) of anti-BTAF1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1]
Mouse Anti-BTAF1 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120002

Other Notes

Small volumes of anti-BTAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTAF1 purchase

MBS120002 is an antibody targeting BTAF1. Also known as Mouse monoclonal antibody Anti-Human BTAF1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BTAF1. It may not necessarily be applicable to this product.

NCBI GI #
50345994
NCBI GeneID
9044
NCBI Accession #
NP_003963.1
UniProt Secondary Accession #
O43578; B4E0W6
UniProt Related Accession #
O14981
GenBank Nucleotide #
NM_003972
NCBI Official Full Name
Homo sapiens BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (BTAF1), mRNA
NCBI Official Synonym Full Names
BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
NCBI Official Symbol
BTAF1
NCBI Official Synonym Symbols
MOT1; TAF172; TAFII170; TAF(II)170
UniProt Protein Name
TATA-binding protein-associated factor 172
UniProt Synonym Protein Names
ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172; TAF-172
Protein Family
TATA-binding protein-associated factor
UniProt Gene Name
BTAF1
UniProt Entry Name
BTAF1_HUMAN
NCBI Protein Information
TATA-binding protein-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); TBP-associated factor 172

NCBI Summary for BTAF1

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]
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UniProt Comments for BTAF1

TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family.

Protein type: Helicase; EC 3.6.4.-; EC 3.6.1.-; Transcription initiation complex

Chromosomal Location of Human Ortholog: 10q22-q23

Cellular Component: nucleus

Molecular Function: DNA binding; helicase activity; transcription factor activity; ATP binding

Biological Process: metabolic process; negative regulation of transcription, DNA-dependent
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Research Articles on BTAF1

1. Results support a model for a BTAF1-mediated release of TBP-NC2 complexes from chromatin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BTAF1 antibody

 Reference Product  PubMed Publications
 TBP antibody  >15 publications with BTAF1 and TBP
 DR1 antibody  >1 publications with BTAF1 and DR1
 DRAP1 antibody  >1 publications with BTAF1 and DRAP1

Diseases associated with anti-BTAF1 antibody

 Disease Name  Pubmed Publications
 Kidney Neoplasms Antibodies  >1 publications with BTAF1 and Kidney Neoplasms
 Kidney Diseases Antibodies  >1 publications with BTAF1 and Kidney Diseases

Organs/Tissues associated with anti-BTAF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >1 publications with BTAF1 and Nerve
 Ear Antibodies  >1 publications with BTAF1 and Ear
 Lymph Node Antibodies  >1 publications with BTAF1 and Lymph Node
 Skin Antibodies  >1 publications with BTAF1 and Skin
 Kidney Antibodies  >1 publications with BTAF1 and Kidney
 Heart Antibodies  >1 publications with BTAF1 and Heart
 Spleen Antibodies  >1 publications with BTAF1 and Spleen
 Blood Antibodies  >1 publications with BTAF1 and Blood
 Uncharacterized Tissue Antibodies  >1 publications with BTAF1 and Uncharacterized Tissue
 Bone Antibodies  >1 publications with BTAF1 and Bone
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