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ACTB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120059

Beta actin is one of six different actin isoforms which have been identified. ACTB is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major… More details

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Product Overview

Online Datasheet in PDF
Product Name
ACTB, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human ACTB
Product Gene Name
anti-ACTB antibody
Product Synonym Names
Homo sapiens actin, beta; PS1TP5BP1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ACTBD11B7
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
41,737 Da
OMIM
102630
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-ACTB antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-ACTB antibody

Western blot analysis of immunized recombinant protein, using anti-ACTB monoclonal antibody.
Mouse Anti-ACTB Monoclonal Antibody – Cat# MBS120059

Quality Control #2 of anti-ACTB antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-ACTB Monoclonal Antibody, Quality Control #2 – Cat# MBS120059

Western Blot (WB) of anti-ACTB antibody

Detection of ACTB by Western blot.

Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. ACTBD11B7-2]

Predicted molecular weight: 41 kDa
Mouse Anti-ACTB Monoclonal Antibody, Western Blot (WB) – Cat# MBS120059

Flow Cytometry (FC/FACS) of anti-ACTB antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ACTB mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. ACTBD11B7-1]
Mouse Anti-ACTB Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120059

Immunocytochemistry (ICC) of anti-ACTB antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ACTB mAb. [Lot No. ACTBD11B7-1]
Mouse Anti-ACTB Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120059

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS120059 is an antibody targeting ACTB. Also known as Mouse monoclonal antibody Anti-Human ACTB. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
33988640
NCBI GeneID
60
NCBI Accession #
BC002409
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
NCBI Official Full Name
Homo sapiens actin, beta, mRNA
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
Protein Family
Actin
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1; PS1TP5-binding protein 1; beta cytoskeletal actin

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTB

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; ribonucleoprotein complex; cytosol; nucleoplasm; cytoskeleton; membrane; axon; nuclear chromatin; cytoplasm; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; 'de novo' posttranslational protein folding; retinal homeostasis; cellular protein metabolic process; substantia nigra development; ATP-dependent chromatin remodeling; ephrin receptor signaling pathway; innate immune response; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1
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Research Articles on ACTB

1. The results indicate that the disease-related human beta-actin mutations p.R183W and p.E364K affect interdomain mobility and nucleotide interactions as a basis for the formation of disease phenotypes in patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ACTB antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram

Diseases associated with anti-ACTB antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >51 publications with ACTB and Carcinoma
 Liver Diseases Antibodies  >23 publications with ACTB and Liver Diseases
 Disease Models, Animal Antibodies  >21 publications with ACTB and Disease Models, Animal
 Kidney Diseases Antibodies  >17 publications with ACTB and Kidney Diseases
 Necrosis Antibodies  >16 publications with ACTB and Necrosis
 Inflammation Antibodies  >14 publications with ACTB and Inflammation
 Liver Neoplasms Antibodies  >12 publications with ACTB and Liver Neoplasms
 Heart Diseases Antibodies  >11 publications with ACTB and Heart Diseases
 Lung Neoplasms Antibodies  >11 publications with ACTB and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >10 publications with ACTB and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-ACTB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >77 publications with ACTB and Blood
 Brain Antibodies  >57 publications with ACTB and Brain
 Liver Antibodies  >55 publications with ACTB and Liver
 Muscle Antibodies  >52 publications with ACTB and Muscle
 Kidney Antibodies  >36 publications with ACTB and Kidney
 Lung Antibodies  >31 publications with ACTB and Lung
 Bone Antibodies  >28 publications with ACTB and Bone
 Heart Antibodies  >26 publications with ACTB and Heart
 Connective Tissue Antibodies  >21 publications with ACTB and Connective Tissue
 Embryonic Tissue Antibodies  >20 publications with ACTB and Embryonic Tissue
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