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PAX8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120074

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PAX8, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human PAX8
Product Gene Name
anti-PAX8 antibody
Product Synonym Names
paired box gene 8
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PAX8R1
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
31,044 Da
OMIM
167415
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-PAX8 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-PAX8 antibody

Western blot analysis of immunized recombinant protein, using anti-PAX8 monoclonal antibody.
Mouse Anti-PAX8 Monoclonal Antibody – Cat# MBS120074

Quality Control #2 of anti-PAX8 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-PAX8 Monoclonal Antibody, Quality Control #2 – Cat# MBS120074

Western Blot (WB) of anti-PAX8 antibody

Detection of PAX8 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. PAX8R1-3]

Predicted molecular weight: 48 kDa
Mouse Anti-PAX8 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120074

Immunoprecipitation (IP) of anti-PAX8 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-PAX8 mAb. [Lot No. PAX8R1-3]

Predicted molecular weight: 48 kDa
Mouse Anti-PAX8 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120074

Flow Cytometry (FC/FACS) of anti-PAX8 antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PAX8 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. PAX8R1-3]
Mouse Anti-PAX8 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120074

Immunocytochemistry (ICC) of anti-PAX8 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PAX8 mAb. [Lot No. PAX8R1-3]
Mouse Anti-PAX8 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120074

Other Notes

Small volumes of anti-PAX8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAX8 purchase

MBS120074 is an antibody targeting PAX8. Also known as Mouse monoclonal antibody Anti-Human PAX8. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PAX8. It may not necessarily be applicable to this product.

NCBI GI #
33987990
NCBI GeneID
7849
NCBI Accession #
BC001060
UniProt Secondary Accession #
Q09155; Q16337; Q16338; Q16339; Q4ZG35; Q96J49
UniProt Related Accession #
Q06710
NCBI Official Full Name
Homo sapiens paired box 8, mRNA
NCBI Official Synonym Full Names
paired box 8
NCBI Official Symbol
PAX8
UniProt Protein Name
Paired box protein Pax-8
Protein Family
Paired box protein
UniProt Gene Name
PAX8
UniProt Entry Name
PAX8_HUMAN
NCBI Protein Information
paired box protein Pax-8; paired domain gene 8

NCBI Summary for PAX8

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
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UniProt Comments for PAX8

PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; thyroid-stimulating hormone receptor activity

Biological Process: regulation of apoptosis; inner ear morphogenesis; anatomical structure morphogenesis; central nervous system development; transcription, DNA-dependent; ureteric bud branching; positive regulation of transcription, DNA-dependent; thyroid gland development; mesonephros development; positive regulation of transcription from RNA polymerase II promoter; kidney development; pronephros development; urogenital system development

Disease: Hypothyroidism, Congenital, Nongoitrous, 2
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Research Articles on PAX8

1. We reviewed the reliability of PAX8 to determine tumor type or primary site in 135 current clinical pelvic or abdominal lesions

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PAX8 antibody

 Reference Product  PubMed Publications
 PPARG antibody  >88 publications with PAX8 and PPARG
 NKX2-1 antibody  >40 publications with PAX8 and NKX2-1
 WT1 antibody  >36 publications with PAX8 and WT1
 RXRA antibody  >1 publications with PAX8 and RXRA
 RB1 antibody  >1 publications with PAX8 and RB1

Pathways associated with anti-PAX8 antibody

 Products by Pathway  Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 TSH Signaling Pathway antibodies  TSH Signaling Pathway Diagram
 Thyroid Cancer Pathway antibodies  Thyroid Cancer Pathway Diagram
 Thyroid Cancer Pathway antibodies  Thyroid Cancer Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-PAX8 antibody

 Disease Name  Pubmed Publications
 Thyroid Diseases Antibodies  >301 publications with PAX8 and Thyroid Diseases
 Thyroid cancer, follicular Antibodies  >139 publications with PAX8 and Thyroid cancer, follicular
 Kidney Diseases Antibodies  >78 publications with PAX8 and Kidney Diseases
 Neoplasm Metastasis Antibodies  >34 publications with PAX8 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >29 publications with PAX8 and Breast Neoplasms
 Lung Diseases Antibodies  >22 publications with PAX8 and Lung Diseases
 Hyperplasia Antibodies  >21 publications with PAX8 and Hyperplasia
 Death Antibodies  >21 publications with PAX8 and Death
 Necrosis Antibodies  >17 publications with PAX8 and Necrosis
 Neoplasms, Experimental Antibodies  >14 publications with PAX8 and Neoplasms, Experimental

Organs/Tissues associated with anti-PAX8 antibody

 Organ/Tissue Name  Pubmed Publications
 Thyroid Antibodies  >494 publications with PAX8 and Thyroid
 Kidney Antibodies  >151 publications with PAX8 and Kidney
 Brain Antibodies  >56 publications with PAX8 and Brain
 Lung Antibodies  >47 publications with PAX8 and Lung
 Ovary Antibodies  >27 publications with PAX8 and Ovary
 Lymph Node Antibodies  >26 publications with PAX8 and Lymph Node
 Bladder Antibodies  >20 publications with PAX8 and Bladder
 Eye Antibodies  >16 publications with PAX8 and Eye
 Cervix Antibodies  >12 publications with PAX8 and Cervix
 Pancreas Antibodies  >12 publications with PAX8 and Pancreas
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