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CDC5L Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120141

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC5L, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human CDC5L
Product Gene Name
anti-CDC5L antibody
Product Synonym Names
Homo sapiens CDC5 cell division cycle 5-like (S. pombe) (CDC5L); CEF1; hCDC5; PCDC5RP; KIAA0432; dJ319D22.1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2136C1a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
92,251 Da
OMIM
602868
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-CDC5L antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-CDC5L antibody

Western blot analysis of immunized recombinant protein, using anti-CDC5L monoclonal antibody.
Mouse Anti-CDC5L Monoclonal Antibody – Cat# MBS120141

Quality Control #2 of anti-CDC5L antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-CDC5L Monoclonal Antibody, Quality Control #2 – Cat# MBS120141

Western Blot (WB) of anti-CDC5L antibody

Detection of CDC5L by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa
Mouse Anti-CDC5L Monoclonal Antibody, Western Blot (WB) – Cat# MBS120141

Immunoprecipitation (IP) of anti-CDC5L antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CDC5L mAb. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa
Mouse Anti-CDC5L Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120141

Flow Cytometry (FC/FACS) of anti-CDC5L antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2136C1a-1]
Mouse Anti-CDC5L Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS120141

Immunocytochemistry (ICC) of anti-CDC5L antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDC5L mAb. [Lot No. 2136C1a-1]
Mouse Anti-CDC5L Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120141

Other Notes

Small volumes of anti-CDC5L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC5L purchase

MBS120141 is an antibody targeting CDC5L. Also known as Mouse monoclonal antibody Anti-Human CDC5L. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC5L. It may not necessarily be applicable to this product.

NCBI GI #
356640202
NCBI GeneID
988
NCBI Accession #
NP_001244.1
UniProt Secondary Accession #
Q76N46; Q99974
UniProt Related Accession #
Q99459
GenBank Nucleotide #
NM_001253
NCBI Official Full Name
Homo sapiens cell division cycle 5-like (CDC5L), mRNA
NCBI Official Synonym Full Names
cell division cycle 5-like
NCBI Official Symbol
CDC5L
NCBI Official Synonym Symbols
CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
UniProt Protein Name
Cell division cycle 5-like protein
UniProt Synonym Protein Names
Pombe cdc5-related protein
Protein Family
Cell division cycle 5-like protein
UniProt Gene Name
CDC5L
UniProt Entry Name
CDC5L_HUMAN
NCBI Protein Information
cell division cycle 5-like protein; CDC5 cell division cycle 5-like; Cdc5-related protein; dJ319D22.1 (CDC5-like protein); pombe cdc5-related protein

UniProt Comments for CDC5L

CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas.

Protein type: Spliceosome; RNA processing; DNA-binding; RNA splicing; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; gene expression; DNA repair
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Research Articles on CDC5L

1. The N-terminal ARM-repeat domain of CTNNBL1 provides a binding site for CDC5L, a binding partner in the Prp19-CDC5L complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC5L antibody

 Reference Product  PubMed Publications
 PLRG1 antibody  >10 publications with CDC5L and PLRG1
 PRPF19 antibody  >9 publications with CDC5L and PRPF19
 CTNNBL1 antibody  >6 publications with CDC5L and CTNNBL1
 PPP1R8 antibody  >2 publications with CDC5L and PPP1R8
 BCAS2 antibody  >2 publications with CDC5L and BCAS2

Pathways associated with anti-CDC5L antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, 35S U5-snRNP Pathway antibodies  Spliceosome, 35S U5-snRNP Pathway Diagram
 Spliceosome, 35S U5-snRNP Pathway antibodies  Spliceosome, 35S U5-snRNP Pathway Diagram
 Spliceosome, Prp19/CDC5L Complex Pathway antibodies  Spliceosome, Prp19/CDC5L Complex Pathway Diagram
 Spliceosome, Prp19/CDC5L Complex Pathway antibodies  Spliceosome, Prp19/CDC5L Complex Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram

Diseases associated with anti-CDC5L antibody

 Disease Name  Pubmed Publications
 Chromosome Aberrations Antibodies  >4 publications with CDC5L and Chromosome Aberrations
 Necrosis Antibodies  >3 publications with CDC5L and Necrosis
 Carcinoma Antibodies  >2 publications with CDC5L and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >1 publications with CDC5L and Cell Transformation, Neoplastic
 Carcinoma, Hepatocellular Antibodies  >1 publications with CDC5L and Carcinoma, Hepatocellular
 Inflammation Antibodies  >1 publications with CDC5L and Inflammation

Organs/Tissues associated with anti-CDC5L antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >5 publications with CDC5L and Bone
 Blood Antibodies  >3 publications with CDC5L and Blood
 Muscle Antibodies  >2 publications with CDC5L and Muscle
 Kidney Antibodies  >2 publications with CDC5L and Kidney
 Embryonic Tissue Antibodies  >2 publications with CDC5L and Embryonic Tissue
 Liver Antibodies  >2 publications with CDC5L and Liver
 Heart Antibodies  >1 publications with CDC5L and Heart
 Eye Antibodies  >1 publications with CDC5L and Eye
 Ovary Antibodies  >1 publications with CDC5L and Ovary
 Uncharacterized Tissue Antibodies  >1 publications with CDC5L and Uncharacterized Tissue
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