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EIF4ENIF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120274

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic;its… More details

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Product Overview

Online Datasheet in PDF
Product Name
EIF4ENIF1, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human EIF4ENIF1
Product Gene Name
Product Synonym Names
Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1); 4E-T; Clast4; FLJ21601
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2314C3a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
108,329 Da
OMIM
607445
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-EIF4ENIF1 antibody
Dot Blot (DB), Immunocytochemistry (ICC)

Quality Control of anti-EIF4ENIF1 antibody

Western blot analysis of immunized recombinant protein, using anti-EIF4ENIF1 monoclonal antibody.
Mouse Anti-EIF4ENIF1 Monoclonal Antibody – Cat# MBS120274

Quality Control #2 of anti-EIF4ENIF1 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-EIF4ENIF1 Monoclonal Antibody, Quality Control #2 – Cat# MBS120274

Immunocytochemistry (ICC) of anti-EIF4ENIF1 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-EIF4ENIF1 mAb. [Lot No.2314C3a-1]
Mouse Anti-EIF4ENIF1 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120274

Other Notes

Small volumes of anti-EIF4ENIF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF4ENIF1 purchase

MBS120274 is an antibody targeting EIF4ENIF1. Also known as Mouse monoclonal antibody Anti-Human EIF4ENIF1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4ENIF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E nuclear import factor 1
NCBI Official Symbol
EIF4ENIF1
NCBI Official Synonym Symbols
4E-T; Clast4
UniProt Protein Name
Eukaryotic translation initiation factor 4E transporter
UniProt Synonym Protein Names
Eukaryotic translation initiation factor 4E nuclear import factor 1
UniProt Gene Name
EIF4ENIF1
UniProt Entry Name
4ET_HUMAN
NCBI Protein Information
eukaryotic translation initiation factor 4E transporter; 2610509L04Rik; eIF4E transporter; eIF4E-transporter

NCBI Summary for EIF4ENIF1

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
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UniProt Comments for EIF4ENIF1

4E-T: Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. Interacts with EIF4E. Interacts with importin beta only in the presence of importin alpha, suggesting a direct interaction with importin alpha. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear import; Translation; Karyopherin

Chromosomal Location of Human Ortholog: 22q11.2

Cellular Component: PML body; intracellular membrane-bound organelle; membrane; cytoplasm; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; protein transporter activity

Biological Process: protein transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF4ENIF1 antibody

Diseases associated with anti-EIF4ENIF1 antibody

Organs/Tissues associated with anti-EIF4ENIF1 antibody

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