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ICOS Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8120214

Background: Inducible costimulator (ICOS), also called AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule) is a cell-surface receptor and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4, and PD-1. The… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ICOS, Active Protein
Full Product Name
ICOS Protein, Human, Recombinant (His & hFc Tag)
Product Gene Name
ICOS active protein
Product Synonym Names
Human ICOS/AILIM/CD278 Protein (His & Fc Tag); AILIM Protein, Human; CD278 Protein, Human; CVID1 Protein, Human; inducible T-cell co-stimulator
Sequence
Met1-Phe14 1
Host
HEK293 Cells
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Purity/Purification
>90% as determined by SDS-PAGE
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Molecular Weight
19,127 Da
OMIM
gene 607594
3D Structure
ModBase 3D Structure for Q9Y6W8
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Activity
Measured by its binding ability in a functional ELISA. Immobilized human human B7-H2 at 1ug/ml (100uL/well) can bind human ICOS with a linear range of 1.6-200ng/mL.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu 21
Tag
C-human IgG1-Fc & His
Protein Construction
A DNA sequence encoding the extracellular domain of human ICOS (NP_036224.1)(Met 1-Phe 141) was fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Product Categories/Family for ICOS active protein

Cancer Drug Targets

SDS-Page of ICOS active protein

ICOS Active Protein – Cat# MBS8120214

Testing Data of ICOS active protein

Measured by its binding ability in a functional ELISA. Immobilized human human B7-H2 at 1 ug/ml (100 ul/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.
ICOS Active Protein, Testing Data – Cat# MBS8120214

Product References and Citations for ICOS active protein

Coyle AJ, et al. (2004) The role of ICOS and other costimulatory molecules in allergy and asthma. Springer Semin Immunopathol. 25(3-4): 349-59.Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.van Berkel ME, et al. (2006) CD28 and ICOS: similar or separate costimulators of T cells Immunol Lett. 105(2): 115-22.

Other Notes

Small volumes of ICOS active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ICOS purchase

MBS8120214 is an active protein targeting ICOS. Also known as ICOS Protein, Human, Recombinant (His & hFc Tag). Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for ICOS. It may not necessarily be applicable to this product.

NCBI GI #
15029518
NCBI GeneID
29851
NCBI Accession #
NP_036224.1
UniProt Primary Accession #
Q9Y6W8
UniProt Secondary Accession #
Q8N6W8
UniProt Related Accession #
Q9Y6W8
GenBank Nucleotide #
NM_012092.3
NCBI Official Full Name
inducible T-cell costimulator
NCBI Official Synonym Full Names
inducible T cell costimulator
NCBI Official Symbol
ICOS
NCBI Official Synonym Symbols
AILIM; CD278; CVID1
UniProt Protein Name
Inducible T-cell costimulator
UniProt Synonym Protein Names
Activation-inducible lymphocyte immunomediatory molecule; CD_antigen: CD278
Protein Family
Inducible T-cell costimulator
UniProt Gene Name
ICOS
UniProt Synonym Gene Names
AILIM
UniProt Entry Name
ICOS_HUMAN
NCBI Protein Information
inducible T-cell costimulator

NCBI Summary for ICOS

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

UniProt Comments for ICOS

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1
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Research Articles on ICOS

1. we have found an association between chronic spontaneous urticaria and polymorphisms in the ICOS gene. To our knowledge, this report is the fi rst to suggest and initially indicate a possible association of the ICOS rs6726035 TT genotype with CSU pathogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ICOS active protein

 Reference Product  PubMed Publications
 CTLA4 active protein  >202 publications with ICOS and CTLA4
 ICOSLG active protein  >60 publications with ICOS and ICOSLG
 RC3H1 active protein  >17 publications with ICOS and RC3H1
 PIK3CD active protein  >5 publications with ICOS and PIK3CD
 CD40LG active protein  >5 publications with ICOS and CD40LG
 PIK3CB active protein  >2 publications with ICOS and PIK3CB
 PIK3CG active protein  >1 publications with ICOS and PIK3CG

Pathways associated with ICOS active protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Constitutive Signaling By Aberrant PI3K In Cancer Pathway antibodies  Constitutive Signaling By Aberrant PI3K In Cancer Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Of Signal Transduction Pathway antibodies  Diseases Of Signal Transduction Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram

Diseases associated with ICOS active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >150 publications with ICOS and Inflammation
 Death Antibodies  >109 publications with ICOS and Death
 Disease Models, Animal Antibodies  >108 publications with ICOS and Disease Models, Animal
 Necrosis Antibodies  >87 publications with ICOS and Necrosis
 Respiratory Tract Diseases Antibodies  >78 publications with ICOS and Respiratory Tract Diseases
 Hypersensitivity Antibodies  >68 publications with ICOS and Hypersensitivity
 Lung Diseases Antibodies  >59 publications with ICOS and Lung Diseases
 Common Variable Immunodeficiency Antibodies  >45 publications with ICOS and Common Variable Immunodeficiency
 Respiratory Hypersensitivity Antibodies  >44 publications with ICOS and Respiratory Hypersensitivity
 Liver Diseases Antibodies  >20 publications with ICOS and Liver Diseases

Organs/Tissues associated with ICOS active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >817 publications with ICOS and Blood
 Lung Antibodies  >62 publications with ICOS and Lung
 Heart Antibodies  >41 publications with ICOS and Heart
 Brain Antibodies  >29 publications with ICOS and Brain
 Thymus Antibodies  >21 publications with ICOS and Thymus
 Pharynx Antibodies  >9 publications with ICOS and Pharynx
 Thyroid Antibodies  >8 publications with ICOS and Thyroid
 Uterus Antibodies  >2 publications with ICOS and Uterus
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