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ZW10 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS120277

The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced… More details

$575 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZW10, Monoclonal Antibody
Full Product Name
Mouse monoclonal antibody Anti-Human ZW10
Product Gene Name
anti-ZW10 antibody
Product Synonym Names
Homo sapiens ZW10, kinetochore associated, homolog (Drosophila) (ZW10); HZW10; KNTC1AP; MGC149821
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3363C4a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Molecular Weight
76,807 Da
OMIM
603954
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Applications Tested/Suitable for anti-ZW10 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)

Quality Control of anti-ZW10 antibody

Western blot analysis of immunized recombinant protein, using anti-ZW10 monoclonal antibody.
Mouse Anti-ZW10 Monoclonal Antibody – Cat# MBS120277

Quality Control #2 of anti-ZW10 antibody

Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Mouse Anti-ZW10 Monoclonal Antibody, Quality Control #2 – Cat# MBS120277

Western Blot (WB) of anti-ZW10 antibody

Detection of ZW10 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3363C4a-1]

Predicted molecular weight: 88 kDa
Mouse Anti-ZW10 Monoclonal Antibody, Western Blot (WB) – Cat# MBS120277

Immunoprecipitation (IP) of anti-ZW10 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ZW10 mAb. [Lot No. 3363C4a-1]

Predicted molecular weight: 88 kDa
Mouse Anti-ZW10 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS120277

Immunocytochemistry (ICC) of anti-ZW10 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZW10 mAb. [Lot No. 3363C4a-1]
Mouse Anti-ZW10 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS120277

Other Notes

Small volumes of anti-ZW10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZW10 purchase

MBS120277 is an antibody targeting ZW10. Also known as Mouse monoclonal antibody Anti-Human ZW10. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ZW10. It may not necessarily be applicable to this product.

NCBI GI #
342837718
NCBI GeneID
9183
NCBI Accession #
NP_004715.1
UniProt Secondary Accession #
A1A528
UniProt Related Accession #
O43264
GenBank Nucleotide #
NM_004724
NCBI Official Full Name
Homo sapiens zw10 kinetochore protein (ZW10), mRNA
NCBI Official Synonym Full Names
zw10 kinetochore protein
NCBI Official Symbol
ZW10
NCBI Official Synonym Symbols
HZW10; KNTC1AP
UniProt Protein Name
Centromere/kinetochore protein zw10 homolog
Protein Family
Centromere/kinetochore protein
UniProt Gene Name
ZW10
UniProt Entry Name
ZW10_HUMAN
NCBI Protein Information
centromere/kinetochore protein zw10 homolog; ZW10 homolog, centromere/kinetochore protein; ZW10, kinetochore associated, homolog; zeste white 10 homolog

NCBI Summary for ZW10

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. [provided by RefSeq, Aug 2011]

UniProt Comments for ZW10

ZW10: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. Belongs to the ZW10 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: kinetochore; kinetochore microtubule; spindle pole; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol

Molecular Function: centromeric DNA binding; protein binding

Biological Process: meiosis; protein transport; ER to Golgi vesicle-mediated transport; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; cell division; mitotic cell cycle checkpoint; regulation of exit from mitosis; protein complex assembly; mitotic cell cycle; Golgi organization and biogenesis; mitotic metaphase plate congression
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Research Articles on ZW10

1. hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZW10 antibody

 Reference Product  PubMed Publications
 ZWILCH antibody  >25 publications with ZW10 and ZWILCH
 ZWINT antibody  >15 publications with ZW10 and ZWINT
 RINT1 antibody  >11 publications with ZW10 and RINT1
 NDC80 antibody  >6 publications with ZW10 and NDC80
 KNTC1 antibody  >2 publications with ZW10 and KNTC1
 INCENP antibody  >1 publications with ZW10 and INCENP

Pathways associated with anti-ZW10 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram
 Mitotic Metaphase And Anaphase Pathway antibodies  Mitotic Metaphase And Anaphase Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
 Resolution Of Sister Chromatid Cohesion Pathway antibodies  Resolution Of Sister Chromatid Cohesion Pathway Diagram
 Separation Of Sister Chromatids Pathway antibodies  Separation Of Sister Chromatids Pathway Diagram

Diseases associated with anti-ZW10 antibody

 Disease Name  Pubmed Publications
 Abnormalities, Drug-Induced Antibodies  >1 publications with ZW10 and Abnormalities, Drug-Induced
 Osteosarcoma Antibodies  >1 publications with ZW10 and Osteosarcoma

Organs/Tissues associated with anti-ZW10 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with ZW10 and Brain
 Embryonic Tissue Antibodies  >2 publications with ZW10 and Embryonic Tissue
 Blood Antibodies  >1 publications with ZW10 and Blood
 Testis Antibodies  >1 publications with ZW10 and Testis
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