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Sensor Protein Kinase Walk (Walk) (SaurJH1_0019) Staphylococcus Aureus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1264819

MBS1264819 is a recombinant protein targeting SaurJH1_0019. Also known as Recombinant Staphylococcus aureus Sensor protein kinase walK (walK). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Sensor protein kinase walK (walK) (SaurJH1_0019), Recombinant Protein
Full Product Name
Recombinant Staphylococcus aureus Sensor protein kinase walK (walK)
Product Synonym Gene Name
walK
Product Synonym Names
Recombinant Sensor protein kinase walK (walK); Sensor protein kinase walK EC= 2.7.13.3
Sequence
MKWLKQLQSL HTKLVIVYVL LIIIGMQIIG LYFTNNLEKE LLDNFKKNIT QYAKQLEISI EKVYDEKGSV NAQKDIQNLL SEYANRQEIG EIRFIDKDQI IIATTKQSNR SLINQKANDS SVQKALSLGQ SNDHLILKDY GGGKDRVWVY NIPVKVDKKV IGNIYIESKI NDVYNQLNNI NQIFIVGTAI SLLITVILGF FIARTITKPI TDMRNQTVEM SRGNYTQRVK IYGNDEIGEL ALAFNNLSKR VQEAQANTES EKRRLDSVIT HMSDGIIATD RRGRIRIVND MALKMLGMAK EDIIGYYMLS VLSLEDEFKL EEIQENNDSF LLDLNEEEGL IARVNFSTIV QETGFVTGYI AVLHDVTEQQ QVERERREFV ANVSHELRTP LTSMNSYIEA LEEGAWKDEE LAPQFLSVTR EETERMIRLV NDLLQLSKMD NESDQINKEI IDFNMFINKI INRHEMSAKD TTFIRDIPKK TIFTEFDPDK MTQVFDNVIT NAMKYSRGDK RVEFHVKQNP LYNRMTIRIK DNGIGIPINK VDKIFDRFYR VDKARTRKMG GTGLGLAISK EIVEAHNGRI WANSVEGQGT SIFITLPCEV IEDGDWDE
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Molecular Weight
69,924 Da
Sequence Positions
1-608
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Chromosome Location
NC_009632.1 (25772..27598)
3D Structure
ModBase 3D Structure for A6TXG9
Species
Staphylococcus aureus (strain JH1)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SaurJH1_0019 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SaurJH1_0019. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PAS sensor protein
NCBI Official Symbol
SaurJH1_0019
UniProt Protein Name
Sensor protein kinase WalK
UniProt Gene Name
walK
UniProt Entry Name
WALK_STAA2
NCBI Protein Information
PAS sensor protein

UniProt Comments for SaurJH1_0019

Function: Member of the two-component regulatory system WalK/WalR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to WalR

By similarity.

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location: Cell membrane; Multi-pass membrane protein

Potential.

Post-translational modification: Autophosphorylated

By similarity.

Sequence similarities: Contains 1 HAMP domain.Contains 1 histidine kinase domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 1 PAS (PER-ARNT-SIM) domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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