Human DNMT3A Recombinant Protein – Cat# MBS1265409

DNA (Cytosine-5)-Methyltransferase 3a (DNMT3A) Human Recombinant Protein

Name: DNMT3A Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1265409
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1265409

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates… More details

$315 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DNA (cytosine-5)-methyltransferase 3A (DNMT3A), Recombinant Protein

Full Product Name

Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A), partial

Product Gene Name

DNMT3A recombinant protein

Product Synonym Names

DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA

Sequence

KIMYVGDVRS VTQKHIQEWG PFDLVIGGSP CNDLSIVNPA RKGLYEGTGR LFFEFYRLLH DARPKEGDDR PFFWLFENVV AMGVSDKRDI SRFLESNPVM IDAKEVSAAH RARYFWGNLP GMNRPLASTV NDKLELQECL EHGRIAKFSK VRTITTRSNS IKQGKDQHFP VFMNEKEDIL WCTEMERVFG FPVHYTDVSN MSRLARQRLL GRSWSVPVIR HLF

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

Molecular Weight

29.9 kDa

Sequence Positions

680-902. Partial

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

OMIM

602769

3D Structure

ModBase 3D Structure for Q9Y6K1

Product Categories/Family for DNMT3A recombinant protein

Epigenetics and Nuclear Signaling

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for DNMT3A recombinant protein

Cloning, expression and chromosome locations of the human DNMT3 gene family.Xie S., Wang Z., Okano M., Nogami M., Li Y., He W.-W., Okumura K., Li E.Gene 236:87-95(1999) A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.Chen T., Ueda Y., Xie S., Li E.J. Biol. Chem. 277:38746-38754(2002) Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases.Kim G.-D., Ni J., Kelesoglu N., Roberts R.J., Pradhan S.EMBO J. 21:4183-4195(2002) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.Nature 434:724-731(2005)

Other Notes

Small volumes of DNMT3A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNMT3A purchase

MBS1265409 is a recombinant protein targeting DNMT3A. Also known as Recombinant Human DNA (cytosine-5)-methyltransferase 3A (DNMT3A), partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for DNMT3A. It may not necessarily be applicable to this product.

NCBI GI #

12751473

NCBI GeneID

1788

NCBI Accession #

NP_072046.2

UniProt Primary Accession #

Q9Y6K1

UniProt Secondary Accession #

Q86TE8; Q86XF5; Q8IZV0; Q8WXU9; E9PEB8

UniProt Related Accession #

Q9Y6K1

GenBank Nucleotide #

NM_022552.4

NCBI Official Full Name

DNA (cytosine-5)-methyltransferase 3A isoform a

NCBI Official Synonym Full Names

DNA (cytosine-5-)-methyltransferase 3 alpha

NCBI Official Symbol

DNMT3A

NCBI Official Synonym Symbols

TBRS; DNMT3A2; M.HsaIIIA

UniProt Protein Name

DNA (cytosine-5)-methyltransferase 3A

UniProt Synonym Protein Names

DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA

Protein Family

DNA

UniProt Gene Name

DNMT3A

UniProt Synonym Gene Names

Dnmt3a; DNA MTase HsaIIIA; M.HsaIIIA

UniProt Entry Name

DNM3A_HUMAN

NCBI Protein Information

DNA (cytosine-5)-methyltransferase 3A

NCBI Summary for DNMT3A

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for DNMT3A

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.37; Methyltransferase

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: chromosome, pericentric region; cytoplasm; euchromatin; nuclear heterochromatin; nuclear matrix; nucleoplasm; nucleus; XY body

Molecular Function: chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; identical protein binding; metal ion binding; protein binding; unmethylated CpG binding

Biological Process: aging; cytosine methylation within a CG sequence; DNA methylation; DNA methylation during embryonic development; DNA methylation during gametogenesis; DNA methylation on cytosine; establishment and/or maintenance of chromatin architecture; gene expression; genetic imprinting; methylation-dependent chromatin silencing; mitotic cell cycle; negative regulation of gene expression, epigenetic; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; regulation of gene expression, epigenetic; response to cocaine; response to estradiol stimulus; response to ionizing radiation; response to lead ion; response to toxin; response to vitamin A; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; spermatogenesis

Disease: Tatton-brown-rahman Syndrome

Research Articles on DNMT3A

1. DNMT3A mutation is associated with acute myeloid leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DNMT3A recombinant protein

Reference Product	PubMed Publications
DNMT1 recombinant protein	>605 publications with DNMT3A and DNMT1
DNMT3B recombinant protein	>592 publications with DNMT3A and DNMT3B
DNMT3L recombinant protein	>125 publications with DNMT3A and DNMT3L
EZH2 recombinant protein	>77 publications with DNMT3A and EZH2
MYC recombinant protein	>21 publications with DNMT3A and MYC
SUV39H1 recombinant protein	>16 publications with DNMT3A and SUV39H1
EHMT2 recombinant protein	>5 publications with DNMT3A and EHMT2
ZBTB17 recombinant protein	>1 publications with DNMT3A and ZBTB17

Pathways associated with DNMT3A recombinant protein

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
DNA Methylation Pathway antibodies	DNA Methylation Pathway Diagram
Epigenetic Regulation Of Gene Expression Pathway antibodies	Epigenetic Regulation Of Gene Expression Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Methionine Degradation Pathway antibodies	Methionine Degradation Pathway Diagram
Methionine Degradation Pathway antibodies	Methionine Degradation Pathway Diagram

Diseases associated with DNMT3A recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>44 publications with DNMT3A and Disease Models, Animal
Adenocarcinoma Antibodies	>39 publications with DNMT3A and Adenocarcinoma
Breast Neoplasms Antibodies	>36 publications with DNMT3A and Breast Neoplasms
Liver Diseases Antibodies	>32 publications with DNMT3A and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>28 publications with DNMT3A and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>26 publications with DNMT3A and Liver Neoplasms
Lung Neoplasms Antibodies	>24 publications with DNMT3A and Lung Neoplasms
Neoplasms, Experimental Antibodies	>19 publications with DNMT3A and Neoplasms, Experimental
Cardiovascular Diseases Antibodies	>18 publications with DNMT3A and Cardiovascular Diseases
Ovarian Neoplasms Antibodies	>16 publications with DNMT3A and Ovarian Neoplasms

Organs/Tissues associated with DNMT3A recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>201 publications with DNMT3A and Blood
Brain Antibodies	>113 publications with DNMT3A and Brain
Liver Antibodies	>74 publications with DNMT3A and Liver
Lung Antibodies	>44 publications with DNMT3A and Lung
Embryonic Tissue Antibodies	>41 publications with DNMT3A and Embryonic Tissue
Testis Antibodies	>31 publications with DNMT3A and Testis
Muscle Antibodies	>30 publications with DNMT3A and Muscle
Nerve Antibodies	>27 publications with DNMT3A and Nerve
Skin Antibodies	>26 publications with DNMT3A and Skin
Prostate Antibodies	>25 publications with DNMT3A and Prostate