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WAS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127276
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Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
WAS, Polyclonal Antibody
Full Product Name
WAS Polyclonal Antibody
Product Gene Name
anti-WAS antibody
Product Synonym Names
WAS; IMD2; THC; THC1; WASP
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
502
OMIM
phenotype 313900
3D Structure
ModBase 3D Structure for P42768
Species
Human
Immunogen
Recombinant protein of human WAS
Calculated Molecular Weight
53kDa

Product Categories/Family for anti-WAS antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-WAS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-WAS antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-WAS antibody

Western blot analysis of extracts of various cell lines, using WAS Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Rabbit Anti-WAS Polyclonal Antibody – Cat# MBS127276

Immunofluorescence (IF) of anti-WAS antibody

Immunofluorescence analysis of U2OS cells using WAS antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-WAS Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS127276

Other Notes

Small volumes of anti-WAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WAS purchase

MBS127276 is an antibody targeting WAS. Also known as WAS Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WAS. It may not necessarily be applicable to this product.

NCBI GI #
1722836
NCBI GeneID
7454
NCBI Accession #
P42768.4
UniProt Primary Accession #
P42768
UniProt Secondary Accession #
Q9BU11; Q9UNJ9
UniProt Related Accession #
P42768
NCBI Official Full Name
Wiskott-Aldrich syndrome protein
NCBI Official Synonym Full Names
Wiskott-Aldrich syndrome
NCBI Official Symbol
WAS
NCBI Official Synonym Symbols
THC; IMD2; SCNX; THC1; WASP
UniProt Protein Name
Wiskott-Aldrich syndrome protein
Protein Family
Wiskott-Aldrich syndrome protein
UniProt Gene Name
WAS
UniProt Synonym Gene Names
IMD2; WASp
UniProt Entry Name
WASP_HUMAN
NCBI Protein Information
wiskott-Aldrich syndrome protein; eczema-thrombocytopenia; thrombocytopenia 1 (X-linked)

NCBI Summary for WAS

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]
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UniProt Comments for WAS

WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.4-p11.21

Cellular Component: intercellular junction; vesicle membrane; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; phospholipase binding; actin binding; SH3 domain binding; protein kinase binding

Biological Process: epidermis development; T cell activation; actin filament polymerization; regulation of catalytic activity; actin filament-based movement; actin polymerization and/or depolymerization; innate immune response; defense response; protein complex assembly; immune response; endosome transport; blood coagulation; T cell receptor signaling pathway

Disease: Thrombocytopenia 1; Neutropenia, Severe Congenital, X-linked
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Research Articles on WAS

1. WASP deficiency perturbs the homeostasis of B-cell compartment in humans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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