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CD274 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS127807
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This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD274, Polyclonal Antibody
Full Product Name
CD274 Polyclonal Antibody
Product Gene Name
anti-CD274 antibody
Product Synonym Names
B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Purity/Purification
Affinity purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Observed MW: 40-50kDa
Calculated MW: 33kDa
OMIM
605402
3D Structure
ModBase 3D Structure for Q9NZQ7
Cross-Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-50 of human PDL1/CD274 (NP_054862.1).
Category
Primary antibody

Product Categories/Family for anti-CD274 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CD274 antibody
Western Blot (WB), Immunohistochemistry Paraffin (IHC-P),Immunofluorescence (IF); Immunocytochemistry (ICC).
Application Notes for anti-CD274 antibody
WB: 1:100 - 1:500
IHC-P: 1:50 - 1:200
IF/ICC: 1:50 - 1:200

Western Blot (WB) of anti-CD274 antibody

Western blot analysis of extracts of Mouse heart, using PD-L1/CD274 antibody (MBS127807) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Rabbit Anti-CD274 Polyclonal Antibody – Cat# MBS127807

Immunofluorescence of anti-CD274 antibody

Immunofluorescence (IF) analysis of human placenta cells using PD-L1/CD274 Rabbit pAb (MBS127807) at dilution of 1:200 (40x lens).
Blue: DAPI for nuclear staining.
Rabbit Anti-CD274 Polyclonal Antibody, Immunofluorescence – Cat# MBS127807

Other Notes

Small volumes of anti-CD274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD274 purchase

MBS127807 is an antibody targeting CD274. Also known as CD274 Polyclonal Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

NCBI GI #
83287884
NCBI GeneID
29126
NCBI Accession #
NP_054862.1
UniProt Primary Accession #
Q9NZQ7
UniProt Secondary Accession #
Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
UniProt Related Accession #
Q9NZQ7
NCBI Official Full Name
Programmed cell death 1 ligand 1
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Synonym Protein Names
B7 homolog 1; B7-H1; CD_antigen: CD274
Protein Family
Programmed cell death
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1

UniProt Comments for CD274

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction
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Research Articles on CD274

1. The recent development of the checkpoint inhibitors anti-programmed death-1 and anti-programmed death ligand-1 has generated an enormous amount of interest as a potential new anticancer strategy in solid tumors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD274 antibody

 Reference Product  PubMed Publications
 CD80 antibody  >391 publications with CD274 and CD80
 PDCD1 antibody  >330 publications with CD274 and PDCD1
 STAT1 antibody  >12 publications with CD274 and STAT1
 PTEN antibody  >10 publications with CD274 and PTEN

Pathways associated with anti-CD274 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram

Diseases associated with anti-CD274 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >109 publications with CD274 and Inflammation
 Liver Diseases Antibodies  >86 publications with CD274 and Liver Diseases
 Necrosis Antibodies  >74 publications with CD274 and Necrosis
 Nervous System Diseases Antibodies  >70 publications with CD274 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >69 publications with CD274 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >63 publications with CD274 and Adenocarcinoma
 Lung Diseases Antibodies  >45 publications with CD274 and Lung Diseases
 Kidney Diseases Antibodies  >44 publications with CD274 and Kidney Diseases
 Lung Neoplasms Antibodies  >41 publications with CD274 and Lung Neoplasms
 Recurrence Antibodies  >26 publications with CD274 and Recurrence

Organs/Tissues associated with anti-CD274 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >772 publications with CD274 and Blood
 Lymph Node Antibodies  >79 publications with CD274 and Lymph Node
 Lung Antibodies  >71 publications with CD274 and Lung
 Bone Antibodies  >62 publications with CD274 and Bone
 Skin Antibodies  >45 publications with CD274 and Skin
 Brain Antibodies  >31 publications with CD274 and Brain
 Pancreas Antibodies  >28 publications with CD274 and Pancreas
 Placenta Antibodies  >19 publications with CD274 and Placenta
 Stomach Antibodies  >13 publications with CD274 and Stomach
 Mouth Antibodies  >9 publications with CD274 and Mouth
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