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Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3 (ALKBH3) Rat Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1282437

MBS1282437 is a recombinant protein targeting Alkbh3. Also known as Recombinant Rat Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$985 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3), Recombinant Protein
Full Product Name
Recombinant Rat Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (Alkbh3)
Product Synonym Gene Name
Alkbh3
Sequence
MGDKRQRARV QGAWATPTKS QSAARPATPA RSRPSQTPGP SWRSKEQQQC DRRFVFKEPQ LVVRAAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDLK EADWILERLC QDVPWKQRMG IREDITYPQP RLTAWYGELP YTYSRVTMEP NPHWLPVLWT LKSRIEENTG HTFNSLLCNF YRDEKDSVDW HSDDEPSLGS CPVIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD HGTLLIMEGA TQADWQHRVP KEYHSRERRV NLTFRTVYPD PRGAPGDTSA ELPLR
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
34,011 Da
Sequence Positions
1-295, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q5XIC8
Species
Rat
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of Alkbh3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Alkbh3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Official Synonym Full Names
alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase
NCBI Official Symbol
Alkbh3
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Synonym Protein Names
Alkylated DNA repair protein alkB homolog 3
UniProt Gene Name
Alkbh3
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3

UniProt Comments for Alkbh3

Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N1-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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