Human BTK Recombinant Protein – Cat# MBS1291613

Tyrosine-Protein Kinase BTK (BTK) Human Recombinant Protein

Name: BTK Recombinant Protein; Tyrosine-Protein Kinase BTK; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1291613
Price: 385 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1291613

MBS1291613 is a recombinant protein targeting BTK. Also known as Recombinant Human Tyrosine-protein kinase BTK (BTK). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$385 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tyrosine-protein kinase BTK (BTK), Recombinant Protein

Full Product Name

Recombinant Human Tyrosine-protein kinase BTK (BTK)

Product Gene Name

BTK recombinant protein

Product Synonym Names

Agammaglobulinemia tyrosine kinaseShort name:ATKB-cell progenitor kinaseShort name:BPKBruton tyrosine kinaseAGMX1

Sequence

MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGRRG SKKGSIDVEK ITCVETVVPE KNPPPERQIP RRGEESSEME QISIIERFPY PFQVVYDEGP LYVFSPTEEL RKRWIHQLKN VIRYNSDLVQ KYHPCFWIDG QYLCCSQTAK NAMGCQILEN RNGSLKPGSS HRKTKKPLPP TPEEDQILKK PLPPEPAAAP VSTSELKKVV ALYDYMPMNA NDLQLRKGDE YFILEESNLP WWRARDKNGQ EGYIPSNYVT EAEDSIEMYE WYSKHMTRSQ AEQLLKQEGK EGGFIVRDSS KAGKYTVSVF AKSTGDPQGV IRHYVVCSTP QSQYYLAEKH LFSTIPELIN YHQHNSAGLI SRLKYPVSQQ NKNAPSTAGL GYGSWEIDPK DLTFLKELGT GQFGVVKYGK WRGQYDVAIK MIKEGSMSED EFIEEAKVMM NLSHEKLVQL YGVCTKQRPI FIITEYMANG CLLNYLREMR HRFQTQQLLE MCKDVCEAME YLESKQFLHR DLAARNCLVN DQGVVKVSDF GLSRYVLDDE YTSSVGSKFP VRWSPPEVLM YSKFSSKSDI WAFGVLMWEI YSLGKMPYER FTNSETAEHI AQGLRLYRPH LASEKVYTIM YSCWHEKADE RPTFKILLSN ILDVMDEES

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Molecular Weight

80kDa

Sequence Positions

1-659aa, Full Length

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

Chromosome Location

Chromosome: X; NC_000023.10 (100604435..100641212, complement). Location: Xq21.33-q22

OMIM

300300

3D Structure

ModBase 3D Structure for Q06187

Species

Homo sapiens (Human)

Subcellular Location

Cytoplasm, Cell membrane, Peripheral membrane protein, Nucleus

Protein Families

Protein kinase superfamily, Tyr protein kinase family, TEC subfamily

Tissue Specificity

Predominantly expressed in B-lymphocytes.

Pathway

NF-kappaB signaling pathway

Relevance

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Product Categories/Family for BTK recombinant protein

Immunology

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Product References and Citations for BTK recombinant protein

"The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases."Vetrie D., Vorechovsky I., Sideras P., Holland J., Davies A., Flinter F., Hammarstroem L., Kinnon C., Levinsky R.J., Bobrow M., Smith C.I.E., Bentley D.R.Nature 361:226-233(1993)
https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:1133
https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=159494
https://www.genome.jp/dbget-bin/www_bget?hsa:695
https://string-db.org/network/9606.ENSP00000308176
https://www.omim.org/entry/300300300300300300

Other Notes

Small volumes of BTK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BTK. It may not necessarily be applicable to this product.

NCBI GI #

4557377

NCBI GeneID

695

NCBI Accession #

NP_000052.1

UniProt Primary Accession #

Q06187

UniProt Secondary Accession #

Q32ML5

UniProt Related Accession #

Q06187

GenBank Nucleotide #

NM_000061.2

NCBI Official Full Name

tyrosine-protein kinase BTK

NCBI Official Synonym Full Names

Bruton agammaglobulinemia tyrosine kinase

NCBI Official Symbol

BTK

NCBI Official Synonym Symbols

AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1

UniProt Protein Name

Tyrosine-protein kinase BTK

UniProt Synonym Protein Names

Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase

Protein Family

Tyrosine-protein kinase

UniProt Gene Name

BTK

UniProt Synonym Gene Names

AGMX1; ATK; BPK; ATK; BPK

UniProt Entry Name

BTK_HUMAN

NCBI Protein Information

tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase

NCBI Summary for BTK

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq, Nov 2008]

UniProt Comments for BTK

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked

Research Articles on BTK

1. study reveals the existence of Btk-dependent molecular cooperation between FcgammaRIIa and TLR4 signaling cascades in LPS-"primed" human neutrophils

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BTK recombinant protein

Reference Product	PubMed Publications
TEC recombinant protein	>190 publications with BTK and TEC
SYK recombinant protein	>154 publications with BTK and SYK
LYN recombinant protein	>117 publications with BTK and LYN
ITK recombinant protein	>82 publications with BTK and ITK
BMX recombinant protein	>48 publications with BTK and BMX
CD19 recombinant protein	>36 publications with BTK and CD19
BLNK recombinant protein	>35 publications with BTK and BLNK
TLR4 recombinant protein	>18 publications with BTK and TLR4
CBL recombinant protein	>15 publications with BTK and CBL
FAS recombinant protein	>13 publications with BTK and FAS

Pathways associated with BTK recombinant protein

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies	Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
BCR Signaling Pathway antibodies	BCR Signaling Pathway Diagram
Class I PI3K Signaling Events Pathway antibodies	Class I PI3K Signaling Events Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram

Diseases associated with BTK recombinant protein

Disease Name	Pubmed Publications
X-linked agammaglobulinemia (XLA); X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) Antibodies	> publications with BTK and X-linked agammaglobulinemia (XLA); X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD)

Organs/Tissues associated with BTK recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>601 publications with BTK and Blood
Bone Antibodies	>107 publications with BTK and Bone
Bone Marrow Antibodies	>95 publications with BTK and Bone Marrow
Spleen Antibodies	>84 publications with BTK and Spleen
Lung Antibodies	>22 publications with BTK and Lung
Liver Antibodies	>20 publications with BTK and Liver
Skin Antibodies	>18 publications with BTK and Skin
Lymph Node Antibodies	>16 publications with BTK and Lymph Node
Heart Antibodies	>16 publications with BTK and Heart
Brain Antibodies	>12 publications with BTK and Brain