Rabbit Anti-POLA2 Polyclonal Antibody – Cat# MBS129285

Western blot analysis of extracts of various cell lines, using POLA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

POLA2 Antibody – Rabbit Polyclonal

Name: POLA2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS129285
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS129285

MBS129285 is an antibody targeting POLA2. Also known as POLA2 Polyclonal Antibody. Reactive in Human. Host: Rabbit. More details

$225 / 0.02 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

POLA2, Polyclonal Antibody

Full Product Name

POLA2 Polyclonal Antibody

Product Gene Name

anti-POLA2 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Concentration

1mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

598

3D Structure

ModBase 3D Structure for Q14181

Species

Human

Immunogen

Recombinant protein of human POLA2

Calculated Molecular Weight

70kDa

Product Categories/Family for anti-POLA2 antibody

Polyclonal

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-POLA2 antibody

Western Blot (WB)

Application Notes for anti-POLA2 antibody

WB: 1:1000 - 1:2000

Other Notes

Small volumes of anti-POLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLA2. It may not necessarily be applicable to this product.

NCBI GI #

90110415

NCBI GeneID

23649

NCBI Accession #

Q14181.2

UniProt Primary Accession #

Q14181

UniProt Secondary Accession #

Q9BPV3; B4DNB4

UniProt Related Accession #

Q14181

NCBI Official Full Name

DNA polymerase alpha subunit B

NCBI Official Synonym Full Names

polymerase (DNA directed), alpha 2, accessory subunit

NCBI Official Symbol

POLA2

UniProt Protein Name

DNA polymerase alpha subunit B

UniProt Synonym Protein Names

DNA polymerase alpha 70 kDa subunit

Protein Family

DNA polymerase

UniProt Gene Name

POLA2

UniProt Entry Name

DPOA2_HUMAN

NCBI Protein Information

DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit; polymerase (DNA-directed), alpha (70kD); polymerase (DNA directed), alpha 2 (70kD subunit)

UniProt Comments for POLA2

POLA2: a DNA polymerase that may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery. DNA polymerase alpha-primase is a four subunit enzyme (subunits A, B, C and D), which is assembled throughout the cell cycle. The largest subunit (subunit A) has DNA polymerase activity, the two smallest subunits (subunits C and D) have DNA primase activity. Subunit B binds to subunit A. Phosphorylated in a cell cycle-dependent manner, in G2/M phase.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; DNA replication

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; cytoplasm

Molecular Function: DNA binding; protein heterodimerization activity; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; protein import into nucleus, translocation; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Research Articles on POLA2

1. Findings indicate that tethering of primase to the replisome by DNA polymerase alpha (pol alpha) is critical for the normal action of DNA replication forks in eukaryotic cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLA2 antibody

Reference Product	PubMed Publications
POLD1 antibody	>2 publications with POLA2 and POLD1
POLA1 antibody	>2 publications with POLA2 and POLA1

Pathways associated with anti-POLA2 antibody

Products by Pathway	Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram
DNA Replication Pre-Initiation Pathway antibodies	DNA Replication Pre-Initiation Pathway Diagram
DNA Polymerase Alpha / Primase Complex Pathway antibodies	DNA Polymerase Alpha / Primase Complex Pathway Diagram
DNA Polymerase Alpha / Primase Complex Pathway antibodies	DNA Polymerase Alpha / Primase Complex Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram

Organs/Tissues associated with anti-POLA2 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with POLA2 and Lung