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TUFM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS129710
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This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been… More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TUFM, Polyclonal Antibody
Full Product Name
TUFM Polyclonal Antibody
Product Gene Name
anti-TUFM antibody
Product Synonym Names
P43; EFTU; COXPD4; EF-TuMT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
452
OMIM
gene 610678
3D Structure
ModBase 3D Structure for P49411
Species
Human
Immunogen
Recombinant protein of human TUFM
Calculated Molecular Weight
49kDa

Product Categories/Family for anti-TUFM antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TUFM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-TUFM antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Western Blot (WB) of anti-TUFM antibody

Western blot analysis of extracts of various cell lines, using TUFM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Rabbit Anti-TUFM Polyclonal Antibody – Cat# MBS129710

Immunohistochemistry (IHC) of anti-TUFM antibody

Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody at dilution of 1:100 (40x lens).
Rabbit Anti-TUFM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS129710

Immunofluorescence (IF) of anti-TUFM antibody

Immunofluorescence analysis of U2OS cells using TUFM antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-TUFM Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS129710

Immunoprecipitation (IP) of anti-TUFM antibody

Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug TUFM antibody. Western blot was performed from the immunoprecipitate using TUFM antibody at a dilition of 1:1000.
Rabbit Anti-TUFM Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS129710

Other Notes

Small volumes of anti-TUFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUFM purchase

MBS129710 is an antibody targeting TUFM. Also known as TUFM Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TUFM. It may not necessarily be applicable to this product.

NCBI GI #
1706611
NCBI GeneID
7284
NCBI Accession #
P49411.2
UniProt Primary Accession #
P49411
UniProt Secondary Accession #
O15276
UniProt Related Accession #
P49411
NCBI Official Full Name
Elongation factor Tu, mitochondrial
NCBI Official Synonym Full Names
Tu translation elongation factor, mitochondrial
NCBI Official Symbol
TUFM
NCBI Official Synonym Symbols
P43; EFTU; COXPD4; EF-TuMT
UniProt Protein Name
Elongation factor Tu, mitochondrial
UniProt Synonym Protein Names
P43
UniProt Gene Name
TUFM
UniProt Synonym Gene Names
EF-Tu
UniProt Entry Name
EFTU_HUMAN
NCBI Protein Information
elongation factor Tu, mitochondrial; EF-Tu

UniProt Comments for TUFM

EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Protein type: RNA-binding; Translation; Translation elongation; Mitochondrial

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: membrane; mitochondrion

Molecular Function: GTPase activity; protein binding; GTP binding; translation elongation factor activity

Biological Process: translational elongation; mitochondrial translation; organelle organization and biogenesis

Disease: Combined Oxidative Phosphorylation Deficiency 4
Show more

Research Articles on TUFM

1. NLRX1 and TUFM work in concert to reduce cytokine response and augment autophagy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUFM antibody

 Reference Product  PubMed Publications
 TSFM antibody  >1 publications with TUFM and TSFM

Pathways associated with anti-TUFM antibody

 Products by Pathway  Pathway Diagram
 Mitochondrial Translation Pathway antibodies  Mitochondrial Translation Pathway Diagram
 Mitochondrial Translation Elongation Pathway antibodies  Mitochondrial Translation Elongation Pathway Diagram
 Organelle Biogenesis And Maintenance Pathway antibodies  Organelle Biogenesis And Maintenance Pathway Diagram

Diseases associated with anti-TUFM antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with TUFM and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with TUFM and Brain Diseases
 Inflammation Antibodies  >3 publications with TUFM and Inflammation
 Musculoskeletal Diseases Antibodies  >2 publications with TUFM and Musculoskeletal Diseases
 Liver Neoplasms Antibodies  >2 publications with TUFM and Liver Neoplasms
 Liver Diseases Antibodies  >1 publications with TUFM and Liver Diseases
 Heart Diseases Antibodies  >1 publications with TUFM and Heart Diseases
 Necrosis Antibodies  >1 publications with TUFM and Necrosis

Organs/Tissues associated with anti-TUFM antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with TUFM and Blood
 Muscle Antibodies  >5 publications with TUFM and Muscle
 Brain Antibodies  >5 publications with TUFM and Brain
 Liver Antibodies  >4 publications with TUFM and Liver
 Lung Antibodies  >4 publications with TUFM and Lung
 Heart Antibodies  >3 publications with TUFM and Heart
 Lymph Antibodies  >2 publications with TUFM and Lymph
 Eye Antibodies  >2 publications with TUFM and Eye
 Placenta Antibodies  >2 publications with TUFM and Placenta
 Spleen Antibodies  >2 publications with TUFM and Spleen
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