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Endoribonuclease Dicer Homolog 1 (DCL1) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1319281

MBS1319281 is a recombinant protein targeting DCL1. Also known as Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 1 (DCL1) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$820 / 0.05 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endoribonuclease Dicer homolog 1 (DCL1), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 1 (DCL1) , partial
Product Gene Name
DCL1 recombinant protein
Product Synonym Gene Name
DCL1
Sequence
PMHPVRELQE RCQQQAEGLE YKASRSGNTA TVEVFIDGVQ VGVAQNPQKK MAQKLAARNA LAALKEKEIA ESKEKHINNG NAGEDQGENE NGNKKNGHQP FTRQTLNDIC LRKNWPMPSY RCVKEGGPAH AKRFTFGVRV NTSDRGWTDE CIGEPMPSVK KAKDSAAVLL LELLNKTFS
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
213,574 Da
Sequence Positions
1731-1909. Fragment at the C-terminal, including the DRBM 1 & DRBM 2 Domain
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q9SP32
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DCL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCL1. It may not necessarily be applicable to this product.

NCBI GI #
15223286
NCBI GeneID
839574
NCBI Accession #
NP_171612.1
UniProt Primary Accession #
Q9SP32
UniProt Secondary Accession #
Q9FDY6; Q9MAN0
UniProt Related Accession #
Q9SP32
GenBank Nucleotide #
NM_099986.4
NCBI Official Full Name
dicer-like 1
NCBI Official Symbol
DCL1
NCBI Official Synonym Symbols
ABNORMAL SUSPENSOR 1; ASU1; ATDCL1; CAF; CARPEL FACTORY; dicer-like 1; DICER-LIKE 1; EMB60; EMB76; EMBRYO DEFECTIVE 60; EMBRYO DEFECTIVE 76; SHORT INTEGUMENTS 1; SIN1; SUS1; SUSPENSOR 1; T25K16.4; T25K16_4
UniProt Protein Name
Endoribonuclease Dicer homolog 1
UniProt Synonym Protein Names
Dicer-like protein 1; AtDCL1; Protein ABNORMAL SUSPENSOR 1; Protein CARPEL FACTORY; Protein SHORT INTEGUMENTS 1; Protein SUSPENSOR 1
Protein Family
Dicer-like protein
UniProt Gene Name
DCL1
UniProt Synonym Gene Names
ASU1; CAF SIN1; SUS1; AtDCL1
NCBI Protein Information
dicer-like 1

NCBI Summary for DCL1

Encodes a Dicer homolog. Dicer is a RNA helicase involved in microRNA processing. Mutations in this locus can result in embryo lethality. Embryo shape at seed maturity is globular-elongate. Other mutants convert the floral meristems to an indeterminate state, others yet show defects in ovule development. mRNA is expressed in all shoot tissues. DCL1 is able to produce miRNAs and siRNAs.
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UniProt Comments for DCL1

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Functions with DRB1/HYL1 and SERRATE proteins for accurate pri-miRNAs to miRNAs processing. Indirectly involved in the production of trans-acting small interfering RNAs (ta-siRNAs) derived from the TAS1, TAS2 or TAS3 endogenous transcripts by participating in the production of their initiating miRNAs. Involved in the processing of natural siRNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving 24 nucleotide nat-siRNAs into 21 nucleotide nat-siRNAs. Can produce RDR6-dependent endogenous ta-siRNAs derived from TAS1 and TAS2. Required for the production of 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). Acts redundantly with DICER-LIKE 3 (DCL3) to promote flowering via repression of FLOWERING LOCUS C (FLC). Represses antiviral RNA silencing through negative regulation of the expression of DCL4 and DCL3.
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Research Articles on DCL1

1. Further analysis revealed that the steady state level of HYL1 protein in plants under osmotic stress is dependent on the SnRK2 kinases. Additionally, our results suggest that the SnRK2 kinases physically associate with the miRNA processing components SE and HYL1 and can phosphorylate these proteins in vitro.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with DCL1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Leaf Antibodies  >23 publications with DCL1 and Leaf
 Flower Antibodies  >8 publications with DCL1 and Flower
 Whole Plant Antibodies  >6 publications with DCL1 and Whole Plant
 Inflorescence Antibodies  >4 publications with DCL1 and Inflorescence
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