Fibrinogen Total Antigen Assay (FGA) ELISA Kit for General

Intended Uses: This rabbit fibrinogen antigen assay is intended for the quantitative determination of total fibrinogen antigen in rabbit plasma and serum. For research use only.

Principle of the Assay: Rabbit fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-rat fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.

Background: Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade [1]. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation [2]. Elevated fibrinogen levels are correlated with cardiovascular disease [3] and atherosclerosis [4].

Small volumes of FGA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS135501 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fibrinogen total antigen assay (FGA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGA. The ELISA analytical biochemical technique of the MBS135501 kit is based on FGA antibody-FGA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).