Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Human Recombinant Protein

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: cell surface; cytoplasm; early endosome; endoplasmic reticulum; extracellular space; extrinsic to external side of plasma membrane; Golgi apparatus; late endosome; lysosome; perinuclear region of cytoplasm; plasma membrane

Molecular Function: apolipoprotein binding; apolipoprotein receptor binding; low-density lipoprotein binding; low-density lipoprotein receptor binding; protein binding; protein self-association; receptor inhibitor activity; serine-type endopeptidase activity; sodium channel inhibitor activity; very-low-density lipoprotein binding

Biological Process: cellular response to insulin stimulus; cellular response to starvation; cholesterol homeostasis; kidney development; liver development; low-density lipoprotein receptor catabolic process; lysosomal transport; negative regulation of receptor recycling; neurogenesis; neuron differentiation; positive regulation of low-density lipoprotein receptor catabolic process; positive regulation of neuron apoptosis; positive regulation of receptor internalization; protein autoprocessing; regulation of neuron apoptosis; regulation of receptor activity

Disease: Hypercholesterolemia, Autosomal Dominant, 3