Human MIA Active Protein – Cat# MBS142187

Melanoma Inhibitory Activity (MIA) Human Active Protein

Name: MIA Active Protein; Melanoma Inhibitory Activity; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142187
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142187

Description: Melanoma Inhibitory Activity Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton.The MIA is purified by proprietary chromatographic… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Melanoma Inhibitory Activity (MIA), Active Protein

Full Product Name

Recombinant Human Melanoma Inhibitory Activity Protein

Product Gene Name

MIA active protein

Product Synonym Names

MIA Human; Melanoma Inhibitory Activity Human Recombinant; Melanoma-derived growth regulatory protein precursor; Cartilage-derived retinoic acid-sensitive protein; CD-RAP; MIA

Sequence

Agrees with the sequence of native MIA human with an addition N-terminal Methionine residue.MG PMPKLADRKL CADQECSSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS LKGRGRFLWG GSVQGDYYGD LAARLGYFPS SIVREDQTLK VDVKTDKWDF YCQ

Host

E Coli

Form/Format

The protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Potassium-phosphate pH=7 and 150mM potassium chloride.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized MIA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MIA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Molecular Weight

6,361 Da

OMIM

601340

3D Structure

ModBase 3D Structure for Q16674

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Melanoma Inhibitory Activity in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

UV spectroscopy at 280 nm using the absorption coefficient of 19300 M-1cm-1.

Biological Activity

The biological activity is calculated by the inhibiting effect on the invasion of Mel In Tumor cells and found active in Mel In assay.

Product Categories/Family for MIA active protein

Cytokines and Growth Factors; Growth Factors; Melanoma Inhibitory Activity

Other Notes

Small volumes of MIA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIA purchase

MBS142187 is an active protein targeting MIA. Also known as Recombinant Human Melanoma Inhibitory Activity Protein. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MIA. It may not necessarily be applicable to this product.

NCBI GI #

321400133

NCBI GeneID

8190

NCBI Accession #

NP_001189482.1

UniProt Primary Accession #

Q16674

UniProt Secondary Accession #

Q6FHV3

UniProt Related Accession #

Q16674

GenBank Nucleotide #

NM_001202553.1

NCBI Official Full Name

melanoma-derived growth regulatory protein

NCBI Official Synonym Full Names

melanoma inhibitory activity

NCBI Official Symbol

MIA

NCBI Official Synonym Symbols

CD-RAP

UniProt Protein Name

Melanoma-derived growth regulatory protein

UniProt Synonym Protein Names

Melanoma inhibitory activity protein

Protein Family

Melanoma-derived growth regulatory protein

UniProt Gene Name

MIA

UniProt Entry Name

MIA_HUMAN

NCBI Protein Information

melanoma-derived growth regulatory protein

UniProt Comments for MIA

MIA: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Belongs to the MIA/OTOR family.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity

Biological Process: cell proliferation

Research Articles on MIA

1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIA active protein

Reference Product	PubMed Publications
TGFB1 active protein	>9 publications with MIA and TGFB1
HMG1 active protein	>5 publications with MIA and HMG1
HMGB1 active protein	>5 publications with MIA and HMGB1
SPP1 active protein	>5 publications with MIA and SPP1
IGF1 active protein	>1 publications with MIA and IGF1
CDH7 active protein	>1 publications with MIA and CDH7

Pathways associated with MIA active protein

Products by Pathway	Pathway Diagram
Neural Crest Differentiation Pathway antibodies	Neural Crest Differentiation Pathway Diagram

Diseases associated with MIA active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>128 publications with MIA and Inflammation
Necrosis Antibodies	>98 publications with MIA and Necrosis
Neoplasms, Experimental Antibodies	>78 publications with MIA and Neoplasms, Experimental
Lung Neoplasms Antibodies	>67 publications with MIA and Lung Neoplasms
Liver Neoplasms Antibodies	>61 publications with MIA and Liver Neoplasms
Breast Neoplasms Antibodies	>58 publications with MIA and Breast Neoplasms
Kidney Diseases Antibodies	>58 publications with MIA and Kidney Diseases
Atherosclerosis Antibodies	>52 publications with MIA and Atherosclerosis
Liver Diseases Antibodies	>44 publications with MIA and Liver Diseases
Heart Diseases Antibodies	>39 publications with MIA and Heart Diseases

Organs/Tissues associated with MIA active protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>120 publications with MIA and Lung
Skin Antibodies	>107 publications with MIA and Skin
Heart Antibodies	>68 publications with MIA and Heart
Lymph Node Antibodies	>50 publications with MIA and Lymph Node
Prostate Antibodies	>47 publications with MIA and Prostate
Stomach Antibodies	>25 publications with MIA and Stomach
Placenta Antibodies	>18 publications with MIA and Placenta
Embryonic Tissue Antibodies	>18 publications with MIA and Embryonic Tissue
Eye Antibodies	>14 publications with MIA and Eye
Ear Antibodies	>7 publications with MIA and Ear