Human G CSF Active Protein – Cat# MBS142206

Granulocyte-Colony Stimulating Factor (G CSF) Human Active Protein

Name: G CSF Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142206
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142206

Description: Granulocyte Colony Stimulating Factor Human Recombinant produced in CHO cells is a single, glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of approximately 18 kDa.G-CSF is purified by proprietary… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Granulocyte-Colony Stimulating Factor (G CSF), Active Protein

Full Product Name

Recombinant Human Granulocyte-Colony Stimulating Factor, CHO

Product Gene Name

G CSF active protein

Product Synonym Names

G CSF Human, CHO; Granulocyte-Colony Stimulating Factor Human Recombinant, CHO; CSF-3; MGI-1G; GM-CSF beta; Pluripoietin; Filgrastim; Lenograstim; G-CSF; MGC45931; GCSF; G CSF CHO

Sequence

TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

Host

Chinese Hamster Ovarian Cells (CHO)

Form/Format

G-CSF was lyophilized from a concentrated (1mg/ml) Phosphate- Buffered Saline, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 97.0% as determined by: a) Analysis by RP-HPLC. b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution G-CSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

18,434 Da

OMIM

138970

3D Structure

ModBase 3D Structure for P09919

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

GCSF quantitation was carried out by two independent methods:1) UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2) Analysis by RP-HPLC, using a calibrated solution of Granulocyte Colony Stimulating Factor as a Reference Standard.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.

Product Categories/Family for G CSF active protein

Cytokines and Growth Factors; Growth Factors; Colony Stimulating Factor

Other Notes

Small volumes of G CSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G CSF purchase

MBS142206 is an active protein targeting G CSF. Also known as Recombinant Human Granulocyte-Colony Stimulating Factor, CHO. Host: Chinese Hamster Ovarian Cells (CHO).

Additional Details

NCBI/Uniprot data below describe general gene information for G CSF. It may not necessarily be applicable to this product.

NCBI GI #

4503079

NCBI GeneID

1440

NCBI Accession #

NP_000750.1

UniProt Primary Accession #

P09919

UniProt Secondary Accession #

A8MXR7

UniProt Related Accession #

P09919

GenBank Nucleotide #

NM_000759.3

NCBI Official Full Name

granulocyte colony-stimulating factor isoform a

NCBI Official Synonym Full Names

colony stimulating factor 3 (granulocyte)

NCBI Official Symbol

CSF3

NCBI Official Synonym Symbols

GCSF; CSF3OS; C17orf33

UniProt Protein Name

Granulocyte colony-stimulating factor

UniProt Synonym Protein Names

Pluripoietin; INN: Filgrastim; INN: Lenograstim

Protein Family

Granulocyte colony-stimulating factor

UniProt Gene Name

CSF3

UniProt Synonym Gene Names

C17orf33; GCSF; G-CSF

UniProt Entry Name

CSF3_HUMAN

NCBI Protein Information

granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin

NCBI Summary for G CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

UniProt Comments for G CSF

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: granulocyte differentiation; positive regulation of protein binding; positive regulation of myeloid cell differentiation; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter

Research Articles on G CSF

1. G-CSF constrains cancer to grow and progress by, respectively, supporting the survival of sympathetic nerve fibers in 6-hydroxydopamine-sympathectomized mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with G CSF active protein

Reference Product	PubMed Publications
CSF3R active protein	>14 publications with G CSF and CSF3R
MMP9 active protein	>7 publications with G CSF and MMP9
IL10 active protein	>6 publications with G CSF and IL10
CXCL12 active protein	>4 publications with G CSF and CXCL12
ERBB2 active protein	>2 publications with G CSF and ERBB2
STAT3 active protein	>2 publications with G CSF and STAT3
SELL active protein	>1 publications with G CSF and SELL
CXCR4 active protein	>1 publications with G CSF and CXCR4

Pathways associated with G CSF active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with G CSF active protein

Disease Name	Pubmed Publications
Disease Susceptibility Antibodies	>6 publications with G CSF and Disease Susceptibility
Nervous System Diseases Antibodies	>5 publications with G CSF and Nervous System Diseases
Breast Neoplasms Antibodies	>3 publications with G CSF and Breast Neoplasms
Neutropenia Antibodies	>3 publications with G CSF and Neutropenia
Leukopenia Antibodies	>2 publications with G CSF and Leukopenia
Anemia Antibodies	>2 publications with G CSF and Anemia
Neoplasm Metastasis Antibodies	>2 publications with G CSF and Neoplasm Metastasis
Leukemia, Myeloid, Acute Antibodies	>2 publications with G CSF and Leukemia, Myeloid, Acute
Peripheral Nervous System Diseases Antibodies	>1 publications with G CSF and Peripheral Nervous System Diseases
Pulmonary Fibrosis Antibodies	>1 publications with G CSF and Pulmonary Fibrosis

Organs/Tissues associated with G CSF active protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>7 publications with G CSF and Skin
Muscle Antibodies	>5 publications with G CSF and Muscle
Lung Antibodies	>5 publications with G CSF and Lung
Brain Antibodies	>3 publications with G CSF and Brain
Liver Antibodies	>2 publications with G CSF and Liver
Uterus Antibodies	>2 publications with G CSF and Uterus
Uncharacterized Tissue Antibodies	>1 publications with G CSF and Uncharacterized Tissue