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Paraoxonase-2 (PON2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142441

Description: Paraoxonase-2 Human Recombinant is expressed in E Coli having a molecular weight of 43.5 kDa and fused to an amino terminal hexahistidine tag.The PON2 purified by proprietary chromatographic techniques.Introduction: Paraoxonase 2 (PON2) is a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Paraoxonase-2 (PON2), Recombinant Protein
Full Product Name
Recombinant Human Paraoxonase-2
Product Gene Name
Product Synonym Names
Serum paraoxonase; arylesterase 2; EC 3.1.1.2; EC 3.1.8.1; PON 2; Serum aryldialkylphosphatase 2; A-esterase 2; Aromatic esterase 2
Sequence
MGRLVAVGLL GIALALLGER LLALRNRLKA SREVESVDLP HCHLIKGIEA GSEDIDILPN GLAFFSVGLK FPGLHSFAPD KPGGILMMDL KEEKPRAREL RISRGFDLAS FNPHGISTFI DNDDTVYLFV VNHPEFKNTV EIFKFEEAEN SLLHLKTVKH ELLPSVNDIT AVGPAHFYAT NDHYFSDPFL KYLETYLNLH WANVVYYSPN EVKVVAEGFD SANGINISPD DKYIYVADIL AHEIHVLEKH TNMNLTQLKV LELDTLVDNL SIDPSSGDIW VGCHPNGQKL FVYDPNNPPS SEVLRIQNIL SEKPTVTTVY ANNGSVLQGS SVASVYDGKL LIGTLYHRAL YCELZ
Host
E Coli
Form/Format
0.1 mg/ml PON2 is supplied in PBS and 50% glycerol.
Sterile Filtered clear solution.
Purity/Purification
Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Molecular Weight
43.5 kDa
OMIM
602447
3D Structure
ModBase 3D Structure for Q15165

Product Categories/Family for PON2 recombinant protein

Applications Tested/Suitable for PON2 recombinant protein
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes for PON2 recombinant protein
Arylesterase 2 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.

Other Notes

Small volumes of PON2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PON2 purchase

MBS142441 is a recombinant protein targeting PON2. Also known as Recombinant Human Paraoxonase-2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PON2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serum paraoxonase/arylesterase 2 isoform 1
NCBI Official Synonym Full Names
paraoxonase 2
NCBI Official Symbol
PON2
UniProt Protein Name
Serum paraoxonase/arylesterase 2
UniProt Synonym Protein Names
Aromatic esterase 2; A-esterase 2; Serum aryldialkylphosphatase 2
UniProt Gene Name
PON2
UniProt Synonym Gene Names
PON 2; A-esterase 2
UniProt Entry Name
PON2_HUMAN
NCBI Protein Information
serum paraoxonase/arylesterase 2; A-esterase 2; PON 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2

NCBI Summary for PON2

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for PON2

PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.81; Motility/polarity/chemotaxis; EC 3.1.1.2; Hydrolase

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; lysosome; extracellular region; plasma membrane; nucleus

Molecular Function: identical protein binding; arylesterase activity; metal ion binding

Biological Process: response to oxidative stress; aromatic compound catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PON2 recombinant protein

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