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Ubiquitin-Conjugating Enzyme E2 35 (UBC35) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1424600

MBS1424600 is a recombinant protein targeting UBC35. Also known as Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 35 (UBC35). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$805 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ubiquitin-conjugating enzyme E2 35 (UBC35), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 35 (UBC35)
Product Gene Name
UBC35 recombinant protein
Product Synonym Gene Name
UBC35
Sequence
MANSNLPRRI IKETQRLLSE PAPGISASPS EDNMRYFNVM ILGPTQSPYE GGVFKLELFL PEEYPMAAPK VRFLTKIYHP NIDKLGRICL DILKDKWSPA LQIRTVLLSI QALLSAPNPD DPLSENIAKH WKSNEAEAVD TAKEWTRLYA SGA
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
12,851 Da
Sequence Positions
1-153, Full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q94A97
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of UBC35 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBC35. It may not necessarily be applicable to this product.

NCBI GI #
79321461
NCBI GeneID
844224
NCBI Accession #
NP_001031298.1
UniProt Primary Accession #
Q94A97
UniProt Secondary Accession #
Q2V4C1; Q9ZVA6
UniProt Related Accession #
Q94A97
GenBank Nucleotide #
NM_001036221.3
NCBI Official Full Name
ubiquitin-conjugating enzyme 35
NCBI Official Symbol
UBC35
NCBI Official Synonym Symbols
F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 35
UniProt Synonym Protein Names
E2 ubiquitin-conjugating enzyme 35; Ubiquitin carrier protein 35
Protein Family
Ubiquitin-conjugating enzyme
UniProt Gene Name
UBC35
UniProt Synonym Gene Names
UBC13A; UBG13A
NCBI Protein Information
ubiquitin-conjugating enzyme 35

NCBI Summary for UBC35

UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.
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UniProt Comments for UBC35

Catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Required for postreplication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.
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Research Articles on UBC35

1. UBC13 activity is critical for all major aspects of root development. The double mutant plants were insensitive to auxin treatments and the ubc13 mutant had a reduced auxin response.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with UBC35 recombinant protein

 Products by Pathway  Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Organs/Tissues associated with UBC35 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Root Antibodies  >1 publications with UBC35 and Root
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