Human PGP9.5 Active Protein – Cat# MBS143126

Ubiquitin Carboxyl-Terminal Hydrolase L1 (PGP9.5) Human Active Protein

Name: PGP9.5 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS143126
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143126

Description: PGP9.5 Human Recombinant full length protein expressed in E Coli, shows a 36 kDa band on SDS-PAGE.The PGP9.5 is purified by proprietary chromatographic techniques.Introduction: UCHL1 (PGP9.5) belongs to a gene family whose products hydrolyze… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Ubiquitin Carboxyl-Terminal Hydrolase L1 (PGP9.5), Active Protein

Full Product Name

Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase L1

Product Gene Name

PGP9.5 active protein

Product Synonym Names

PGP9.5 Human; PGP9.5 Human Recombinant; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; EC 3.4.19.12; Ubiquitin thioesterase L1; Neuron cytoplasmic protein 9.5; PGP 9.5; UCHL1; PGP9.5; PARK5

Sequence

MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA

Host

E Coli

Form/Format

PGP9.5 in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol.
Sterile Filtered clear solution.

Preparation and Storage

Store vial at -20 degree C to -80 degree C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.

Molecular Weight

24,824 Da

OMIM

191342

3D Structure

ModBase 3D Structure for P09936

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.

Product Categories/Family for PGP9.5 active protein

Recombinant & Natural Proteins; Recombinant Proteins; Ubiquitin

Applications Tested/Suitable for PGP9.5 active protein

ELISA (EIA), Inhibition Assays, Western Blot (WB)

Other Notes

Small volumes of PGP9.5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGP9.5 purchase

MBS143126 is an active protein targeting PGP9.5. Also known as Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase L1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PGP9.5. It may not necessarily be applicable to this product.

NCBI GI #

21361091

NCBI GeneID

7345

NCBI Accession #

NP_004172.2

UniProt Primary Accession #

P09936

UniProt Secondary Accession #

Q4W5K6; Q71UM0

UniProt Related Accession #

P09936

GenBank Nucleotide #

NM_004181.4

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Official Synonym Full Names

ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)

NCBI Official Symbol

UCHL1

NCBI Official Synonym Symbols

NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L1

UniProt Synonym Protein Names

Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1

UniProt Gene Name

UCHL1

UniProt Synonym Gene Names

UCH-L1; PGP9.5

UniProt Entry Name

UCHL1_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase isozyme L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin thioesterase L1

NCBI Summary for PGP9.5

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

UniProt Comments for PGP9.5

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Ligase; Ubiquitin conjugating system; Protease; Cell development/differentiation; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: omega peptidase activity; protein binding; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: cell proliferation; negative regulation of MAP kinase activity; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; axon target recognition; axon transport of mitochondrion; eating behavior; neuromuscular process; muscle fiber development; sensory perception of pain; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant

Research Articles on PGP9.5

1. This review summarizes recent findings related to the actions of UCH-L1 in human disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PGP9.5 active protein

Reference Product	PubMed Publications
SNCA active protein	>26 publications with PGP9.5 and SNCA
APP active protein	>8 publications with PGP9.5 and APP
TP53 active protein	>3 publications with PGP9.5 and TP53
HSP90AA1 active protein	>2 publications with PGP9.5 and HSP90AA1
LAMP2 active protein	>1 publications with PGP9.5 and LAMP2
HSPA8 active protein	>1 publications with PGP9.5 and HSPA8

Pathways associated with PGP9.5 active protein

Products by Pathway	Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Parkinson's Disease Pathway antibodies	Parkinson's Disease Pathway Diagram
Parkinsons Disease Pathway antibodies	Parkinsons Disease Pathway Diagram
Proteasome Degradation Pathway antibodies	Proteasome Degradation Pathway Diagram

Diseases associated with PGP9.5 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>215 publications with PGP9.5 and Nervous System Diseases
Brain Diseases Antibodies	>159 publications with PGP9.5 and Brain Diseases
Movement Disorders Antibodies	>99 publications with PGP9.5 and Movement Disorders
Inflammation Antibodies	>47 publications with PGP9.5 and Inflammation
Disease Models, Animal Antibodies	>35 publications with PGP9.5 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>32 publications with PGP9.5 and Cardiovascular Diseases
Peripheral Nervous System Diseases Antibodies	>31 publications with PGP9.5 and Peripheral Nervous System Diseases
Necrosis Antibodies	>23 publications with PGP9.5 and Necrosis
Nerve Degeneration Antibodies	>20 publications with PGP9.5 and Nerve Degeneration
Brain Injuries Antibodies	>17 publications with PGP9.5 and Brain Injuries

Organs/Tissues associated with PGP9.5 active protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>197 publications with PGP9.5 and Nerve
Brain Antibodies	>128 publications with PGP9.5 and Brain
Skin Antibodies	>92 publications with PGP9.5 and Skin
Bone Antibodies	>62 publications with PGP9.5 and Bone
Muscle Antibodies	>60 publications with PGP9.5 and Muscle
Lymph Antibodies	>58 publications with PGP9.5 and Lymph
Lymph Node Antibodies	>57 publications with PGP9.5 and Lymph Node
Vascular Antibodies	>56 publications with PGP9.5 and Vascular
Lung Antibodies	>39 publications with PGP9.5 and Lung
Bone Marrow Antibodies	>37 publications with PGP9.5 and Bone Marrow