Human AKR1C1 Active Protein – Cat# MBS143943

Aldo-Keto Reductase Family 1 Member C1 (AKR1C1) Human Active Protein

Name: AKR1C1 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS143943
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143943

Description: AKR1C1 Human Recombinant fused to 20 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 343 amino acids (1-323 a.a.) and having a molecular mass of 38.9 kDa. The AKR1C1 is purified by… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Aldo-Keto Reductase Family 1 Member C1 (AKR1C1), Active Protein

Full Product Name

Recombinant Human Aldo-Keto Reductase Family 1 Member C1

Product Gene Name

AKR1C1 active protein

Product Synonym Names

AKR1C1 Human; Aldo-Keto Reductase Family 1 Member C1 Human Recombinant; DDH1; DDH; HAKRC; 20-alpha-HSD; DD1/DD2; HBAB; C9; DD1; H-37; MBAB; MGC8954; 2-ALPHA-HSD; AKR1C1; Aldo-keto reductase family 1 member C1; 20-alpha-hydroxysteroid dehydrogenase; Trans-1; 2-dihydrobenzene-1; 2-diol dehydrogenase; Indanol dehydrogenase; Dihydrodiol dehydrogenase 1/2; Chlordecone reductase homolog HAKRC; High-affinity hepatic bile acid-binding protein

Sequence

MGSSHHH HHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Host

E Coli

Form/Format

The AKR1C1 solution contains 20mM Tris-HCl pH-8, 1mM DTT and 20% glycerol.
Sterile Filtered clear colorless solution.

Purity/Purification

Greater than 90% as determined by SDS-PAGE.

Preparation and Storage

Store at 4°C if entire vial will be used within 2-4 weeks.
Store frozen at -20°C for longer periods of time .
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid freeze-thaw cycles.

OMIM

600449

3D Structure

ModBase 3D Structure for Q04828

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is >500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25°C.

Product Categories/Family for AKR1C1 active protein

Enzymes; Reductase

Other Notes

Small volumes of AKR1C1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKR1C1 purchase

MBS143943 is an active protein targeting AKR1C1. Also known as Recombinant Human Aldo-Keto Reductase Family 1 Member C1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR1C1. It may not necessarily be applicable to this product.

NCBI GI #

5453543

NCBI GeneID

1645

NCBI Accession #

NP_001344.2

UniProt Primary Accession #

Q04828

UniProt Secondary Accession #

P52896; Q5SR15; Q7M4N2; Q9UCX2

UniProt Related Accession #

Q04828

GenBank Nucleotide #

NM_001353.5

NCBI Official Full Name

aldo-keto reductase family 1 member C1

NCBI Official Synonym Full Names

aldo-keto reductase family 1, member C1

NCBI Official Symbol

AKR1C1

NCBI Official Synonym Symbols

C9; DD1; DDH; DDH1; H-37; HBAB; MBAB; HAKRC; DD1/DD2; 2-ALPHA-HSD; 20-ALPHA-HSD

UniProt Protein Name

Aldo-keto reductase family 1 member C1

UniProt Synonym Protein Names

20-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.149); 20-alpha-HSD; Chlordecone reductase homolog HAKRC; Dihydrodiol dehydrogenase 1/2; DD1/DD2; High-affinity hepatic bile acid-binding protein; HBAB; Indanol dehydrogenase (EC:1.1.1.112); Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20)

Protein Family

Aldo-keto reductase family

UniProt Gene Name

AKR1C1

UniProt Synonym Gene Names

DDH; DDH1; 20-alpha-HSD; DD1/DD2; HBAB

UniProt Entry Name

AK1C1_HUMAN

NCBI Protein Information

aldo-keto reductase family 1 member C1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase C; chlordecone reductase homolog HAKRC; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; dihydrodiol dehydrogenase isoform DD1; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase

NCBI Summary for AKR1C1

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

UniProt Comments for AKR1C1

AKR1C1: Converts progesterone to its inactive form, 20-alpha- dihydroxyprogesterone (20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May play a role in myelin formation. Belongs to the aldo/keto reductase family.

Protein type: EC 1.1.1.112; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.149; EC 1.3.1.20; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytosol

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldo-keto reductase activity; 20-alpha-hydroxysteroid dehydrogenase activity; indanol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: bile acid and bile salt transport; phototransduction, visible light; response to organophosphorus; cholesterol homeostasis; retinal metabolic process; epithelial cell differentiation; bile acid metabolic process; xenobiotic metabolic process; digestion; cholesterol absorption; progesterone metabolic process; retinoid metabolic process; protein homooligomerization

Research Articles on AKR1C1

1. Studies indicate that mutations in aldo-keto reductase family 1 (AKR1) enzymes AKR1C1 and AKR1C4 are responsible for sexual development dysgenesis and mutations in AKR1D1 are causative in bile-acid deficiency.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AKR1C1 active protein

Reference Product	PubMed Publications
CYP1A1 active protein	>11 publications with AKR1C1 and CYP1A1
EPHX1 active protein	>2 publications with AKR1C1 and EPHX1
CYP1A2 active protein	>2 publications with AKR1C1 and CYP1A2
CYP2B6 active protein	>2 publications with AKR1C1 and CYP2B6
HSD17B3 active protein	>1 publications with AKR1C1 and HSD17B3

Pathways associated with AKR1C1 active protein

Products by Pathway	Pathway Diagram
Benzo(a)pyrene Metabolism Pathway antibodies	Benzo(a)pyrene Metabolism Pathway Diagram
Bile Acid And Bile Salt Metabolism Pathway antibodies	Bile Acid And Bile Salt Metabolism Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
Retinoid Metabolism And Transport Pathway antibodies	Retinoid Metabolism And Transport Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with AKR1C1 active protein

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>17 publications with AKR1C1 and Breast Neoplasms
Adenocarcinoma Antibodies	>13 publications with AKR1C1 and Adenocarcinoma
Endometriosis Antibodies	>11 publications with AKR1C1 and Endometriosis
Lung Neoplasms Antibodies	>10 publications with AKR1C1 and Lung Neoplasms
Liver Neoplasms Antibodies	>6 publications with AKR1C1 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>5 publications with AKR1C1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>4 publications with AKR1C1 and Inflammation
Carcinoma, Non-Small-Cell Lung Antibodies	>4 publications with AKR1C1 and Carcinoma, Non-Small-Cell Lung
Endometrial Neoplasms Antibodies	>3 publications with AKR1C1 and Endometrial Neoplasms
Neoplasms, Experimental Antibodies	>2 publications with AKR1C1 and Neoplasms, Experimental

Organs/Tissues associated with AKR1C1 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>29 publications with AKR1C1 and Liver
Lung Antibodies	>26 publications with AKR1C1 and Lung
Uterus Antibodies	>10 publications with AKR1C1 and Uterus
Ovary Antibodies	>9 publications with AKR1C1 and Ovary
Adipose Tissue Antibodies	>9 publications with AKR1C1 and Adipose Tissue
Connective Tissue Antibodies	>8 publications with AKR1C1 and Connective Tissue
Blood Antibodies	>7 publications with AKR1C1 and Blood
Brain Antibodies	>7 publications with AKR1C1 and Brain
Kidney Antibodies	>7 publications with AKR1C1 and Kidney
Skin Antibodies	>7 publications with AKR1C1 and Skin