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Dcmp Deaminase (DCTD) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS143970

Description: DCTD Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
dCMP Deaminase (DCTD), Recombinant Protein
Full Product Name
Recombinant Human dCMP Deaminase
Product Gene Name
Product Synonym Names
DCTD Human; dCMP Deaminase Human Recombinant; Deoxycytidylate deaminase; EC 3.5.4.12; dCMP Deaminase; DCTD; MGC111062
Sequence
MGSSHHH HHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
Host
E Coli
Form/Format
The DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol.
Sterile filtered colorless solution.
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Molecular Weight
21,014 Da
OMIM
607638
3D Structure
ModBase 3D Structure for P32321

Product Categories/Family for DCTD recombinant protein

Other Notes

Small volumes of DCTD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DCTD purchase

MBS143970 is a recombinant protein targeting DCTD. Also known as Recombinant Human dCMP Deaminase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DCTD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
deoxycytidylate deaminase isoform a
NCBI Official Synonym Full Names
dCMP deaminase
NCBI Official Symbol
DCTD
UniProt Protein Name
Deoxycytidylate deaminase
UniProt Synonym Protein Names
dCMP deaminase
UniProt Gene Name
DCTD
UniProt Entry Name
DCTD_HUMAN
NCBI Protein Information
deoxycytidylate deaminase

NCBI Summary for DCTD

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DCTD

DCTD: catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.5.4.12; Hydrolase

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol

Molecular Function: zinc ion binding; dCMP deaminase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; pyrimidine nucleoside biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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