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Nitrilase Family Member 2 (NIT2) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144243

Description: NIT2 produced in E Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276a.a.) and having a molecular mass of 33kDa.NIT2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nitrilase Family Member 2 (NIT2), Recombinant Protein
Full Product Name
Recombinant Human Nitrilase Family Member 2
Product Gene Name
Product Synonym Names
NIT2 Human; Nitrilase Family Member 2 Human Recombinant; Nitrilase homolog 2; Nitrilase family member 2; omega-amidase NIT2; MGC111199; Nit protein 2; EC 3.5.1.3
Sequence
MGSSHHH HHH SSGLVPRGSH MGSMTS FRLA LIQLQISSIK SDNVTRACSF IREAATQGAK IVSLPECFNS PYGAKYFPEY AEKIPGESTQ KLSEVAKECS IYLIGGSIPE EDAGKLYNTC AVFGPDGTLL AKYRKIHLFD IDVPGKITFQ ESKTLSPGDS FSTFDTPYCR VGLGICYDMR FAELAQIYAQ RGCQLLVYPG AFNLTTGPAH WELLQRSRAV DNQVYVATAS PARDDKASYV AWGHSTVVNP WGEVLAKAGT EEAIVYSDID LKKLAEIRQQ IPVFRQKRSD LYAVEMKKP
Host
E Coli
Form/Format
The NIT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 0.1M NaCl, and 10% glycerol.
Sterile Filtered clear solution.
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Please avoid freeze thaw cycles.
Molecular Weight
30,608 Da
3D Structure
ModBase 3D Structure for Q9NQR4

Product Categories/Family for NIT2 recombinant protein

Other Notes

Small volumes of NIT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NIT2 purchase

MBS144243 is a recombinant protein targeting NIT2. Also known as Recombinant Human Nitrilase Family Member 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NIT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
omega-amidase NIT2
NCBI Official Synonym Full Names
nitrilase family, member 2
NCBI Official Symbol
NIT2
NCBI Official Synonym Symbols
HEL-S-8a
UniProt Protein Name
Omega-amidase NIT2
UniProt Synonym Protein Names
Nitrilase homolog 2
Protein Family
UniProt Gene Name
NIT2
UniProt Entry Name
NIT2_HUMAN
NCBI Protein Information
omega-amidase NIT2; Nit protein 2; epididymis secretory sperm binding protein Li 8a; nitrilase homolog 2

UniProt Comments for NIT2

NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.

Protein type: Mitochondrial; EC 3.5.1.3; Hydrolase

Chromosomal Location of Human Ortholog: 3q12.2

Cellular Component: centrosome; mitochondrion; cytoplasm

Molecular Function: omega-amidase activity

Biological Process: oxaloacetate metabolic process; glutamine metabolic process; asparagine metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NIT2 recombinant protein

 Reference Product  PubMed Publications
 MDH1 recombinant protein  >1 publications with NIT2 and MDH1
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