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Phosphohistidine Phosphatase 1 (PHPT1) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS144317

Description: PHPT1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (1-125a.a.) and having a molecular mass of 15.9kDa.PHPT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Phosphohistidine Phosphatase 1 (PHPT1), Recombinant Protein
Full Product Name
Recombinant Human Phosphohistidine Phosphatase 1
Product Gene Name
Product Synonym Names
PHPT1 Human; Phosphohistidine Phosphatase 1 Human Recombinant; PHP14; CGI-202; HSPC141; Phosphohistidine Phosphatase 1; Phosphohistidine Phosphatase 14kDa; Protein janus-A homolog; Sex-regulated protein Janus-a
Sequence
MGSSHHH HHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Host
E Coli
Form/Format
The PHPT1 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0) 0.2M NaCl, 2mM DTT and 10% glycerol.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protien (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Molecular Weight
13,672 Da
OMIM
610167
3D Structure
ModBase 3D Structure for Q9NRX4

Product Categories/Family for PHPT1 recombinant protein

Other Notes

Small volumes of PHPT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHPT1 purchase

MBS144317 is a recombinant protein targeting PHPT1. Also known as Recombinant Human Phosphohistidine Phosphatase 1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PHPT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
14 kDa phosphohistidine phosphatase isoform 2
NCBI Official Synonym Full Names
phosphohistidine phosphatase 1
NCBI Official Symbol
PHPT1
NCBI Official Synonym Symbols
PHP14; CGI-202; HSPC141; HEL-S-132P
UniProt Protein Name
14 kDa phosphohistidine phosphatase
UniProt Synonym Protein Names
Phosphohistidine phosphatase 1; Protein janus-A homolog
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14
UniProt Entry Name
PHP14_HUMAN
NCBI Protein Information
14 kDa phosphohistidine phosphatase; epididymis secretory sperm binding protein Li 132P; protein janus-A homolog; sex-regulated protein janus-a

NCBI Summary for PHPT1

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for PHPT1

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine; EC 3.1.3.-; Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PHPT1 recombinant protein

 Reference Product  PubMed Publications
 KCNN4 recombinant protein  >2 publications with PHPT1 and KCNN4
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