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ALKB Alkylation Repair Homolog 3 (ALKBH3) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS145072

Description: ALKBH3 Human Recombinant fused to 39 amino acid His Tag at N-terminal produced in E Coli is a single, non-glycosylated, polypeptide chain containing 325 amino acids (1-286 a.a.) and having a molecular mass of 37.9kDa. The ALKBH3 is purified by… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALKB Alkylation Repair Homolog 3 (ALKBH3), Recombinant Protein
Full Product Name
Recombinant Human ALKB Alkylation Repair Homolog 3
Product Gene Name
ALKBH3 recombinant protein
Product Synonym Names
ALKBH3 Human; ALKB Alkylation Repair Homolog 3 Human Recombinant; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; Alkylated DNA repair protein alkB homolog 3; DEPC-1; Prostate cancer antigen 1; ALKBH3; ABH3; DEPC1; PCA1; FLJ43614; MGC118790; MGC118792; MGC118793
Sequence
MRGSHHH HHH GMASMTGGQQ MGRDLYDDDD KDRWGSELD< /u>M EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW
Host
E Coli
Form/Format
The ALKBH3 solution 1mg/ml contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Molecular Weight
19,310 Da
OMIM
610603
3D Structure
ModBase 3D Structure for Q96Q83

Product Categories/Family for ALKBH3 recombinant protein

Enzymes

Other Notes

Small volumes of ALKBH3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALKBH3 purchase

MBS145072 is a recombinant protein targeting ALKBH3. Also known as Recombinant Human ALKB Alkylation Repair Homolog 3. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH3. It may not necessarily be applicable to this product.

NCBI GI #
21040275
NCBI GeneID
221120
NCBI Accession #
NP_631917.1
UniProt Primary Accession #
Q96Q83
UniProt Secondary Accession #
Q3SYI0; Q6NX57; Q96BU8; A6NDJ1
UniProt Related Accession #
Q96Q83
GenBank Nucleotide #
NM_139178.3
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
NCBI Official Synonym Full Names
alkB, alkylation repair homolog 3 (E. coli)
NCBI Official Symbol
ALKBH3
NCBI Official Synonym Symbols
ABH3; PCA1; DEPC1; DEPC-1
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
UniProt Synonym Protein Names
Alkylated DNA repair protein alkB homolog 3; DEPC-1; Prostate cancer antigen 1
Protein Family
Alpha-ketoglutarate-dependent dioxygenase
UniProt Gene Name
ALKBH3
UniProt Synonym Gene Names
ABH3; DEPC1
UniProt Entry Name
ALKB3_HUMAN
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; alkylated DNA repair protein alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1

NCBI Summary for ALKBH3

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ALKBH3

ALKBH3: Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; DNA repair, damage; Hydrolase; EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; L-ascorbic acid binding; ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity

Biological Process: cell proliferation; DNA dealkylation; DNA repair
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Research Articles on ALKBH3

1. ALKBH3 contributes to development of urothelial carcinomas by accelerating their survival, angiogenesis, and invasion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ALKBH3 recombinant protein

 Reference Product  PubMed Publications
 ALKBH1 recombinant protein  >6 publications with ALKBH3 and ALKBH1
 FTO recombinant protein  >2 publications with ALKBH3 and FTO
 ALKBH4 recombinant protein  >1 publications with ALKBH3 and ALKBH4
 ALKBH8 recombinant protein  >1 publications with ALKBH3 and ALKBH8

Pathways associated with ALKBH3 recombinant protein

 Products by Pathway  Pathway Diagram
 ABH3 Mediated Reversal Of Alkylation Damage Pathway antibodies  ABH3 Mediated Reversal Of Alkylation Damage Pathway Diagram
 DNA Damage Reversal Pathway antibodies  DNA Damage Reversal Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Reversal Of Alkylation Damage By DNA Dioxygenases Pathway antibodies  Reversal Of Alkylation Damage By DNA Dioxygenases Pathway Diagram

Diseases associated with ALKBH3 recombinant protein

 Disease Name  Pubmed Publications
 Pancreatic Diseases Antibodies  >2 publications with ALKBH3 and Pancreatic Diseases
 Inflammation Antibodies  >1 publications with ALKBH3 and Inflammation
 Obesity Antibodies  >1 publications with ALKBH3 and Obesity
 Hyperplasia Antibodies  >1 publications with ALKBH3 and Hyperplasia
 Respiratory Tract Diseases Antibodies  >1 publications with ALKBH3 and Respiratory Tract Diseases
 Necrosis Antibodies  >1 publications with ALKBH3 and Necrosis

Organs/Tissues associated with ALKBH3 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >4 publications with ALKBH3 and Prostate
 Bladder Antibodies  >1 publications with ALKBH3 and Bladder
 Pancreas Antibodies  >1 publications with ALKBH3 and Pancreas
 Intestine Antibodies  >1 publications with ALKBH3 and Intestine
 Lung Antibodies  >1 publications with ALKBH3 and Lung
 Brain Antibodies  >1 publications with ALKBH3 and Brain
 Vascular Antibodies  >1 publications with ALKBH3 and Vascular
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