Ribonuclease H1 (RNASEH1) E. Coli Recombinant Protein

This E. coli-expressed RNASEH1 (Ribonuclease H1) recombinant protein is a His-tagged, single-chain polypeptide suited for nucleic acid enzymology and RNA-DNA hybrid research.

Description: RNASEH1 E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (1-155 a.a.) and having a molecular mass of 20kDa.RNASEH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: RNHA is an endonuclease which specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, the RNHA protein mediates the removal of Okazaki fragment RNA primers which are present on the lagging strand during DNA replication. RNHA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is capable of binding magnesium or manganese as cofactors.

Small volumes of RNASEH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

During transcription or DNA replication the DNA-RNA hybridizing strand must be released. The equivalent to a Superman at a molecular level, Ribonuclease H1 breaks these lagging bonds. Many common molecular biology techniques in function genomic studies requires use of recombinant RNAse products and we meet your demanding needs with E coli cell expressed purified recombinant protein products designed by Origene. Each RNASE H1 gene ( RNaseH1 gene ) from species ranging human mouse rat is ready for use in RNA biology experiments, IHC applications such as FISH - FISH probes, ELISA kits control, signaling pathways modifications or for development of drugs compounds screening for diseases domains as suggested by PubMed id 'ed publications. Our recombinant human products proteins section of our expression catalogue will allow creative scientists 360 degree genomic views of possible applications. Order RNaseH 1 h00246243 q01 and a paired monoclonal antibody from MyBiosource.com