Human GOT1 Active Protein – Cat# MBS146772

Glutamic-Oxaloacetic Transaminase 1 (GOT1) Human Active Protein

Name: GOT1 Active Protein; Glutamic-Oxaloacetic Transaminase 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS146772
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS146772

Description: GOT1 Human Recombinant E Coli produced in E Coli is a single, non-glycosylated polypeptide chain containing 433 amino acids (1-413 a.a.) and having a molecular mass of 48.4 kDa. The GOT1 is fused to a 20 amino acid His-Tag at N-terminus and… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Glutamic-Oxaloacetic Transaminase 1 (GOT1), Active Protein

Full Product Name

Recombinant Human Glutamic-Oxaloacetic Transaminase 1, Active

Product Gene Name

GOT1 active protein

Product Synonym Names

GOT1 Human, Active; EC 2.6.1.1; Aspartate aminotransferase 1; Transaminase A; GIG18; Glutamic-Oxaloacetic Transaminase 1 Human Recombinant, Active

Sequence

MG SSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ

Host

E Coli

Form/Format

Sterile filtered colorless solution.
The GOT1 solution (0.5mg/ml) containing 20mM Tris-HCl pH-8.0, 2mM DTT, 0.1M NaCl & 20% glycerol.

Purity/Purification

Greater than 95.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

OMIM

M37400 mRNA

3D Structure

ModBase 3D Structure for P17174

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Product Categories/Family for GOT1 active protein

Enzymes

Other Notes

Small volumes of GOT1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT1 purchase

MBS146772 is an active protein targeting GOT1. Also known as Recombinant Human Glutamic-Oxaloacetic Transaminase 1, Active. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.

NCBI GI #

4504067

NCBI GeneID

2805

NCBI Accession #

NP_002070.1

UniProt Primary Accession #

P17174

UniProt Secondary Accession #

Q5VW80; B2R6R7; B7Z7E9

UniProt Related Accession #

P17174

GenBank Nucleotide #

NM_002079.2

NCBI Official Full Name

aspartate aminotransferase, cytoplasmic

NCBI Official Synonym Full Names

glutamic-oxaloacetic transaminase 1

NCBI Official Symbol

GOT1

NCBI Official Synonym Symbols

AST1; cCAT; GIG18; cAspAT; ASTQTL1

UniProt Protein Name

Aspartate aminotransferase, cytoplasmic

UniProt Synonym Protein Names

Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

Protein Family

Aspartate aminotransferase

UniProt Gene Name

GOT1

UniProt Synonym Gene Names

cAspAT; cCAT

NCBI Protein Information

aspartate aminotransferase, cytoplasmic

NCBI Summary for GOT1

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

UniProt Comments for GOT1

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleus

Molecular Function: aspartate transaminase activity; cysteine transaminase activity; identical protein binding; pyridoxal phosphate binding

Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; gluconeogenesis; glutamate metabolic process; glycerol biosynthetic process; response to glucocorticoid stimulus

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1

Research Articles on GOT1

1. Elevated levels of ALT are Associated with Cardiovascular disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GOT1 active protein

Reference Product	PubMed Publications
GOT2 active protein	>7 publications with GOT1 and GOT2
MDH1 active protein	>5 publications with GOT1 and MDH1
LDHB active protein	>3 publications with GOT1 and LDHB
MDH2 active protein	>2 publications with GOT1 and MDH2

Pathways associated with GOT1 active protein

Products by Pathway	Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
2-Oxocarboxylic Acid Metabolism Pathway antibodies	2-Oxocarboxylic Acid Metabolism Pathway Diagram
Alanine And Aspartate Metabolism Pathway antibodies	Alanine And Aspartate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Alanine, Aspartate And Glutamate Metabolism Pathway antibodies	Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies	Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram

Diseases associated with GOT1 active protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>5 publications with GOT1 and Disease Models, Animal
Nervous System Diseases Antibodies	>4 publications with GOT1 and Nervous System Diseases
Lung Neoplasms Antibodies	>4 publications with GOT1 and Lung Neoplasms
Breast Neoplasms Antibodies	>3 publications with GOT1 and Breast Neoplasms
Cardiovascular Diseases Antibodies	>3 publications with GOT1 and Cardiovascular Diseases
Liver Neoplasms Antibodies	>2 publications with GOT1 and Liver Neoplasms
Liver Diseases Antibodies	>2 publications with GOT1 and Liver Diseases
Fatty Liver Antibodies	>2 publications with GOT1 and Fatty Liver
Fibrosis Antibodies	>2 publications with GOT1 and Fibrosis
Weight Gain Antibodies	>1 publications with GOT1 and Weight Gain

Organs/Tissues associated with GOT1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with GOT1 and Blood
Liver Antibodies	>7 publications with GOT1 and Liver
Nerve Antibodies	>7 publications with GOT1 and Nerve
Intestine Antibodies	>4 publications with GOT1 and Intestine
Lung Antibodies	>4 publications with GOT1 and Lung
Heart Antibodies	>4 publications with GOT1 and Heart
Brain Antibodies	>4 publications with GOT1 and Brain
Muscle Antibodies	>3 publications with GOT1 and Muscle
Vascular Antibodies	>3 publications with GOT1 and Vascular
Thyroid Antibodies	>2 publications with GOT1 and Thyroid