Baculoviral IAP Repeat-Containing Protein 1 (NAIP) Human Recombinant Protein

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that ses to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C. violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C. violaceum and L. pneumophila.

The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy.Roy N., Mahadevan M.S., McLean M., Shutler G., Yaraghi Z., Farahini R., Baird S., Besner-Johnston A., Lefebvre C., Kang X., Salih M., Aubry H., Tamai K., Guan X., Ioannou P., Crawford T.O., de Jong P.J., Surh L., Ikeda J., Korneluk R.G., Mackenzie A.Cell 80:167-178(1995) Sequence of a 131-kb region of 5q13.1 containing the spinal muscular atrophy candidate genes SMN and NAIP.Chen Q., Baird S.D., Mahadevan M., Besner-Johnston A., Farahani R., Xuan J.-Y., Kang X., Lefebvre C., Ikeda J.-E., Korneluk R.G., MacKenzie A.E.Genomics 48:121-127(1998) The DNA sequence and comparative analysis of human chromosome 5.Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S., Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.Nature 431:268-274(2004) Functional human NAIP promoter transcription regulatory elements for the NAIP and PsiNAIP genes.Xu M., Okada T., Sakai H., Miyamoto N., Yanagisawa Y., MacKenzie A.E., Hadano S., Ikeda J.-E.Biochim. Biophys. Acta 1574:35-50(2002) Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes.Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J., Mackenzie A., Korneluk R.G.Nature 379:349-353(1996) The neuronal apoptosis inhibitory protein is a direct inhibitor of caspases 3 and 7.Maier J.K., Lahoua Z., Gendron N.H., Fetni R., Johnston A., Davoodi J., Rasper D., Roy S., Slack R.S., Nicholson D.W., MacKenzie A.E.J. Neurosci. 22:2035-2043(2002) Integrity of ATP binding site is essential for effective inhibition of the intrinsic apoptosis pathway by NAIP.Karimpour S., Davoodi J., Ghahremani M.H.Biochem. Biophys. Res. Commun. 407:158-162(2011) The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus.Zhao Y., Yang J., Shi J., Gong Y.N., Lu Q., Xu H., Liu L., Shao F.Nature 477:596-600(2011) The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with Legionella pneumophila.Katagiri N., Shobuike T., Chang B., Kukita A., Miyamoto H.Microbes Infect. 14:1123-1132(2012) Structures of BIR domains from human NAIP and cIAP2.Herman M.D., Moche M., Flodin S., Welin M., Tresaugues L., Johansson I., Nilsson M., Nordlund P., Nyman T.Acta Crystallogr. F 65:1091-1096(2009)

Small volumes of NAIP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1475860 is a recombinant protein targeting NAIP. Also known as Recombinant Human Baculoviral IAP repeat-containing protein 1. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.