POLR2E Antibody – Rabbit Polyclonal

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; DNA-directed RNA polymerase I complex; DNA-directed RNA polymerase II, core complex; DNA-directed RNA polymerase III complex; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA-directed RNA polymerase activity; protein binding

Biological Process: DNA repair; gene expression; innate immune response; mRNA capping; negative regulation of gene expression, epigenetic; nuclear mRNA splicing, via spliceosome; nucleotide-excision repair; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of viral transcription; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; RNA elongation from RNA polymerase III promoter; RNA splicing; RNA-mediated gene silencing; somatic stem cell maintenance; termination of RNA polymerase I transcription; termination of RNA polymerase III transcription; transcription from RNA polymerase I promoter; transcription from RNA polymerase II promoter; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction