Rabbit Anti-ALKBH8 Polyclonal Antibody – Cat# MBS1497960

Immunohistochemistry of paraffin-embedded human colon cancer using MBS1497960 at dilution 1:100

Alkylated DNA Repair Protein Alkb Homolog 8 (ALKBH8) Antibody – Rabbit Polyclonal

Name: ALKBH8 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1497960
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1497960

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of… More details

$230 / 0.05 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Alkylated DNA repair protein alkB homolog 8 (ALKBH8), Polyclonal Antibody

Full Product Name

Rabbit anti-human Alkylated DNA repair protein alkB homolog 8 polyclonal Antibody(ALKBH8)

Product Gene Name

anti-ALKBH8 antibody

Product Synonym Names

Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; ALKBH8; ABH8

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Antigen Affinity Purified

Preparation and Storage

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Molecular Weight

75,558 Da

OMIM

613306

3D Structure

ModBase 3D Structure for Q96BT7

Storage Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Conjugate

Non-conjugated

Immunogen

Recombinant humanAlkylated DNA repair protein alkB homolog 8 protein (1-230aa)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-ALKBH8 antibody

ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-ALKBH8 antibody

Recommended dilution: IHC:1:20-1:200

Other Notes

Small volumes of anti-ALKBH8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALKBH8 purchase

MBS1497960 is an antibody targeting ALKBH8. Also known as Rabbit anti-human Alkylated DNA repair protein alkB homolog 8 polyclonal Antibody(ALKBH8). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH8. It may not necessarily be applicable to this product.

NCBI GI #

666335573

NCBI GeneID

91801

NCBI Accession #

NP_001287939.1

UniProt Primary Accession #

Q96BT7

UniProt Secondary Accession #

Q8N989; B1Q2M0; B4DEF6

UniProt Related Accession #

Q96BT7

GenBank Nucleotide #

NM_001301010.1

NCBI Official Full Name

alkylated DNA repair protein alkB homolog 8 isoform 1

NCBI Official Synonym Full Names

alkB homolog 8, tRNA methyltransferase

NCBI Official Symbol

ALKBH8

NCBI Official Synonym Symbols

ABH8; TRM9; TRMT9

UniProt Protein Name

Alkylated DNA repair protein alkB homolog 8

UniProt Synonym Protein Names

Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 (EC:2.1.1.229)

Protein Family

Alkylated DNA repair protein

UniProt Gene Name

ALKBH8

UniProt Synonym Gene Names

ABH8

UniProt Entry Name

ALKB8_HUMAN

NCBI Protein Information

alkylated DNA repair protein alkB homolog 8

UniProt Comments for ALKBH8

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.229; RNA processing; Methyltransferase

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: cytosol; microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function: iron ion binding; nucleotide binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; protein binding; tRNA (uracil) methyltransferase activity; tRNA binding; zinc ion binding

Biological Process: gene expression; response to DNA damage stimulus; tRNA methylation; tRNA modification; tRNA processing; tRNA wobble uridine modification

Research Articles on ALKBH8

1. Many eukaryotic tRNAs contain the wobble modification 5-methoxycarbonylmethyl-uridine (mcm5U). It is demonstrated that (R)- and (S)-5-methoxycarbonylhydroxymethyluridine (mchm5U), hydroxylated forms of mcm(5)U, are present in mammalian tRNA-Arg(UCG), and tRNA-Gly(UCC), respectively. It is shown that the hydroxylation reaction leading to the formation of (S)-mchm5U is catalyzed by the oxygenase (AlkB) domain of ALKBH8.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ALKBH8 antibody

Reference Product	PubMed Publications
ALKBH1 antibody	>4 publications with ALKBH8 and ALKBH1
ALKBH2 antibody	>2 publications with ALKBH8 and ALKBH2
ALKBH7 antibody	>1 publications with ALKBH8 and ALKBH7
ALKBH3 antibody	>1 publications with ALKBH8 and ALKBH3

Pathways associated with anti-ALKBH8 antibody

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
TRNA Modification In The Nucleus And Cytosol Pathway antibodies	TRNA Modification In The Nucleus And Cytosol Pathway Diagram
TRNA Processing Pathway antibodies	TRNA Processing Pathway Diagram

Diseases associated with anti-ALKBH8 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>1 publications with ALKBH8 and Carcinoma

Organs/Tissues associated with anti-ALKBH8 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with ALKBH8 and Blood