• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DNA Replication Licensing Factor MCM3 (MCM3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1498914

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through… More details

$230 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA replication licensing factor MCM3, Polyclonal Antibody
Full Product Name
Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)
Product Gene Name
anti-MCM3 antibody
Product Synonym Names
DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102; MCM3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
95,908 Da
OMIM
602693
3D Structure
ModBase 3D Structure for P25205
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human DNA replication licensing factor MCM3 protein (1-295aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9849 / sc-9850
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MCM3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
Application Notes for anti-MCM3 antibody
Recommended dilution: WB:1:200-1000
IHC:1:20-1:200

Western Blot (WB) of anti-MCM3 antibody

All lanes: MCM3 antibody at 4 ug/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4:HepG2 whole cell lysate
Lane 5:Jurkats whole cell lysate
Lane 6:U917 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 91,96 kDa
Observed band size: 91 kDa
Rabbit Anti-MCM3 Polyclonal Antibody – Cat# MBS1498914

Immunohistochemistry (IHC) of anti-MCM3 antibody

Immunohistochemistry of paraffin-embedded human tonsil using MBS1498914 at dilution 1:100
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498914

Immunohistochemistry (IHC) of anti-MCM3 antibody

Immunohistochemistry of paraffin-embedded human liver using MBS1498914 at dilution 1:100
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498914

Immunohistochemistry (IHC) of anti-MCM3 antibody

Immunohistochemistry of paraffin-embedded human colon cancer using MBS1498914 at dilution 1:100
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498914

Immunohistochemistry (IHC) of anti-MCM3 antibody

Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS1498914 at dilution 1:100
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1498914

Chromatin Immunoprecipitation (ChIP) of anti-MCM3 antibody

Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.
Rabbit Anti-MCM3 Polyclonal Antibody, Chromatin Immunoprecipitation (ChIP) – Cat# MBS1498914

Other Notes

Small volumes of anti-MCM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM3 purchase

MBS1498914 is an antibody targeting MCM3. Also known as Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM3. It may not necessarily be applicable to this product.

NCBI GI #
394582099
NCBI GeneID
4172
NCBI Accession #
NP_001257401.1
UniProt Primary Accession #
P25205
UniProt Secondary Accession #
Q92660; Q9BTR3; Q9NUE7; B4DWW4
UniProt Related Accession #
P25205
GenBank Nucleotide #
NM_001270472.1
NCBI Official Full Name
DNA replication licensing factor MCM3 isoform 2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Synonym Protein Names
DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102
Protein Family
DNA replication licensing factor
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN
NCBI Protein Information
DNA replication licensing factor MCM3

NCBI Summary for MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Show more

UniProt Comments for MCM3

MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.

Protein type: EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: alpha DNA polymerase:primase complex; centrosome; intracellular membrane-bound organelle; MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; DNA binding; DNA helicase activity; protein binding

Biological Process: DNA duplex unwinding; DNA replication; DNA replication initiation; DNA strand elongation during DNA replication; G1/S transition of mitotic cell cycle; mitotic cell cycle
Show more

Research Articles on MCM3

1. normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCM3 antibody

 Reference Product  PubMed Publications
 MCM2 antibody  >96 publications with MCM3 and MCM2
 MCM7 antibody  >56 publications with MCM3 and MCM7
 MCM4 antibody  >50 publications with MCM3 and MCM4
 MCM5 antibody  >47 publications with MCM3 and MCM5
 MCM6 antibody  >36 publications with MCM3 and MCM6
 CDC45 antibody  >14 publications with MCM3 and CDC45
 CDK2 antibody  >10 publications with MCM3 and CDK2
 DBF4 antibody  >4 publications with MCM3 and DBF4
 MCMBP antibody  >2 publications with MCM3 and MCMBP

Pathways associated with anti-MCM3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM3 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >8 publications with MCM3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >8 publications with MCM3 and Breast Neoplasms
 Nervous System Diseases Antibodies  >7 publications with MCM3 and Nervous System Diseases
 Skin Neoplasms Antibodies  >7 publications with MCM3 and Skin Neoplasms
 Cell Transformation, Neoplastic Antibodies  >6 publications with MCM3 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >5 publications with MCM3 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >4 publications with MCM3 and Lung Neoplasms
 Precancerous Conditions Antibodies  >4 publications with MCM3 and Precancerous Conditions
 Neoplasm Metastasis Antibodies  >4 publications with MCM3 and Neoplasm Metastasis
 Liver Diseases Antibodies  >3 publications with MCM3 and Liver Diseases

Organs/Tissues associated with anti-MCM3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with MCM3 and Blood
 Skin Antibodies  >9 publications with MCM3 and Skin
 Lymph Node Antibodies  >9 publications with MCM3 and Lymph Node
 Kidney Antibodies  >7 publications with MCM3 and Kidney
 Lung Antibodies  >7 publications with MCM3 and Lung
 Liver Antibodies  >6 publications with MCM3 and Liver
 Brain Antibodies  >6 publications with MCM3 and Brain
 Stomach Antibodies  >6 publications with MCM3 and Stomach
 Lymph Antibodies  >5 publications with MCM3 and Lymph
 Intestine Antibodies  >4 publications with MCM3 and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000