strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: TACE 1131 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 4 Immunocytochemistry Validation of TACE in HeLa Cells
Immunohistochemical analysis of HeLa cells using anti-TACE antibody (1131) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 5 KD Validation of TACE in Monkey COS Cells. (Wang et al., 2006)
COS cells stably expressing Pref-1A were transfected with control siRNA or TACE siRNA. TACE was detected in lysates by using the anti-TACE antibody (1131). TACE expression levels were markedly reduced in TACE knockdown cell lysate.
Figure 6 KD Validation of TACE in MDA-MB-435 Cells. (McGowan et al., 2007)
ADAM-17 protein expression, following transfection with ADAM-17 shRNA (two clones) or neomycin-resistant negative control vector, was examined by immunoblot analysis with anti-ADAM-17 antibodies (1131).
Figure 7 Overexpression Validation of TACE in MCF-7 Cells. (McGowan et al., 2007)
ADAM-17 (TACE) protein expression, following transfection of vector and ADAM-17 cDNA, was examined by immunoblot analysis with anti-ADAM-17 (1131) antibodies in MCF-7 cells. Increased ADAM-17 was detected in ADAM-17 transfected cells.
Figure 8 Induced Expression Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)
Effect of oxygen–glucose deprivation
(OGD) or glutamate on the levels of TACE/ADAM17 in rat cortical cultures. Western blot analysis of TACE in homogenates from control, glutamate, and OGD-exposed cultures from a representative experiment.
Figure 9 Immunofluorescence Validation of TACE in Rat Cortical Neurons (Hurtado et al., 2002)
Double immunostaining of control and glutamate-exposed rat cortical cultures. (A) Control cultures show TACE immunoreactivity at the cellular membrane of some microglial cells (B) Glutamate-exposed cultures show that most microglial cells express TACE immunoreactivity.
(C) Control cultures show that TACE immunostaining does not colocalize with astrocytes [glial fibrillary acidic protein (GFAP)-positive cells]. (D) Astrocyte (GFAP-positive cell) showing TACE immunoreactivity in its surface after treatment with glutamate.
Figure 10 Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)
Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.
ELISA Kit
CLIA Kit
Assay Kit
Antibodies
Polyclonal Antibody
Secondary Antibody
Antigen
Biochemical
cDNA Clone
siRNA
Peptide
Rec./Purified Protein
Custom ELISA Kit
Custom Protein
Custom Antibody
Antibody Matched Pairs
Antibody Peptide Pairs
Phospho Antibodies
Products by Disease
Products by Pathway
Products by Tissue