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ACOT13 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150103

THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
THEM2 (ACOT13), Polyclonal Antibody
Full Product Name
THEM2 Antibody
Product Gene Name
Product Synonym Names
THEM2; HT012; THEM2; PNAS-27; HT012; Acyl-coenzyme A thioesterase 13; Thioesterase superfamily member 2; Acyl-CoA thioesterase 13; acyl-CoA thioesterase 13
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
THEM2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
THEM2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
THEM2 peptide (MBS152029) is used for blocking the activity of THEM2 antibody (MBS150103)
Molecular Weight
12,366 Da
OMIM
615652
3D Structure
ModBase 3D Structure for Q9NPJ3
Conjugate
Unconjugated
Immunogen
THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2.
Buffer
THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-ACOT13 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ACOT13 antibody
THEM2 antibody can be used for detection of THEM2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-ACOT13 antibody

Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-ACOT13 Polyclonal Antibody – Cat# MBS150103

Immunohistochemistry (IHC) of anti-ACOT13 antibody

Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 μg/mL.
Rabbit Anti-ACOT13 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150103

Immunofluorescence (IF) of anti-ACOT13 antibody

Immunofluorescence of THEM2 in human liver tissue with THEM2 antibody at 20 μg/mL.
Rabbit Anti-ACOT13 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150103

Other Notes

Small volumes of anti-ACOT13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACOT13 purchase

MBS150103 is an antibody targeting ACOT13. Also known as THEM2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT13. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-coenzyme A thioesterase 13 isoform 1
NCBI Official Synonym Full Names
acyl-CoA thioesterase 13
NCBI Official Symbol
ACOT13
NCBI Official Synonym Symbols
HT012; THEM2; PNAS-27
UniProt Protein Name
Acyl-coenzyme A thioesterase 13
UniProt Synonym Protein Names
Thioesterase superfamily member 2Acyl-coenzyme A thioesterase 13, N-terminally processed
UniProt Gene Name
ACOT13
UniProt Synonym Gene Names
THEM2; Acyl-CoA thioesterase 13
UniProt Entry Name
ACO13_HUMAN
NCBI Protein Information
acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2

NCBI Summary for ACOT13

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]
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UniProt Comments for ACOT13

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; cytosol; nucleus

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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