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AFAP1L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150104

AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AFAP1L (AFAP1L1), Polyclonal Antibody
Full Product Name
AFAP1L Antibody
Product Gene Name
Product Synonym Names
AFAP1L; Actin filament-associated protein 1-like 1; AFAP1-like protein 1; actin filament associated protein 1-like 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
AFAP1L Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Preparation and Storage
AFAP1L antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
AFAP1L1 peptide (MBS152864) is used for blocking the activity of AFAP1L antibody (MBS150104)
Molecular Weight
43,455 Da
OMIM
614410
3D Structure
ModBase 3D Structure for Q8TED9
Conjugate
Unconjugated
Immunogen
AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.
Buffer
AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-AFAP1L1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-AFAP1L1 antibody
AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-AFAP1L1 antibody

Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.
Rabbit Anti-AFAP1L1 Polyclonal Antibody – Cat# MBS150104

Immunocytochemistry (IHC) of anti-AFAP1L1 antibody

Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.
Rabbit Anti-AFAP1L1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150104

Immunofluorescence (IF) of anti-AFAP1L1 antibody

Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.
Rabbit Anti-AFAP1L1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150104

Other Notes

Small volumes of anti-AFAP1L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AFAP1L1 purchase

MBS150104 is an antibody targeting AFAP1L1. Also known as AFAP1L Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AFAP1L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
actin filament-associated protein 1-like 1 isoform 1
NCBI Official Synonym Full Names
actin filament associated protein 1-like 1
NCBI Official Symbol
AFAP1L1
UniProt Protein Name
Actin filament-associated protein 1-like 1
UniProt Gene Name
AFAP1L1
UniProt Synonym Gene Names
AFAP1-like protein 1
UniProt Entry Name
AF1L1_HUMAN
NCBI Protein Information
actin filament-associated protein 1-like 1; AFAP1-like protein 1

UniProt Comments for AFAP1L1

AFAP1L1: a protein containing two pleckstrin homology (PH) domains, which mediate protein-protein and protein-lipid interactions.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cytoplasm; plasma membrane; podosome; cell junction; nucleus

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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