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JMJD5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150216

JMJD5 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
JMJD5, Polyclonal Antibody
Full Product Name
JMJD5 Antibody
Product Gene Name
Product Synonym Names
JMJD5; JMJD5; JMJD5; Lysine-specific demethylase 8; JmjC domain-containing protein 5; jumonji domain containing 5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
JMJD5 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
JMJD5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
JMJD5 peptide (MBS152843) is used for blocking the activity of JMJD5 antibody (MBS150216)
Molecular Weight
50,852 Da
OMIM
611917
3D Structure
ModBase 3D Structure for Q8N371
Conjugate
Unconjugated
Immunogen
JMJD5 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD5.
Buffer
JMJD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-JMJD5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-JMJD5 antibody
JMJD5 antibody can be used for detection of JMJD5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-JMJD5 antibody

Western blot analysis of JMJD5 in human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-JMJD5 Polyclonal Antibody – Cat# MBS150216

Immunohistochemistry (IHC) of anti-JMJD5 antibody

Immunohistochemistry of JMJD5 in rat liver tissue with JMJD5 antibody at 2.5 μg/mL.
Rabbit Anti-JMJD5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150216

Immunofluorescence (IF) of anti-JMJD5 antibody

Immunofluorescence of JMJD5 in Rat Liver cells with JMJD5 antibody at 20 μg/mL.
Rabbit Anti-JMJD5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150216

Other Notes

Small volumes of anti-JMJD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JMJD5 purchase

MBS150216 is an antibody targeting JMJD5. Also known as JMJD5 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JMJD5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lysine-specific demethylase 8 isoform 1
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 8
NCBI Official Symbol
KDM8
NCBI Official Synonym Symbols
JMJD5
UniProt Protein Name
Lysine-specific demethylase 8
UniProt Synonym Protein Names
JmjC domain-containing protein 5; Jumonji domain-containing protein 5
UniProt Gene Name
KDM8
UniProt Synonym Gene Names
JMJD5
UniProt Entry Name
KDM8_HUMAN
NCBI Protein Information
lysine-specific demethylase 8; jumonji domain containing 5; jmjC domain-containing protein 5; jumonji domain-containing protein 5

NCBI Summary for JMJD5

This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2009]

UniProt Comments for JMJD5

JMJD5: Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; EC 1.14.11.27; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: nucleus

Molecular Function: histone demethylase activity (H3-K36 specific); metal ion binding; chromatin binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JMJD5 antibody

 Reference Product  PubMed Publications
 RCCD1 antibody  >1 publications with JMJD5 and RCCD1

Diseases associated with anti-JMJD5 antibody

Organs/Tissues associated with anti-JMJD5 antibody

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