Apelin 13 (AP13) ELISA Kit for Human

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with AP13. During the reaction, AP13 in the sample or standard competes with a fixed amount of AP13 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to AP13. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AP13 in the samples is then determined by comparing the O D of the samples to the standard curve.

Small volumes of AP13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS762336 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apelin 13 (AP13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP13. The ELISA analytical biochemical technique of the MBS762336 kit is based on AP13 antibody-AP13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).