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GLS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150300

GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes… More details

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Product Overview

Online Datasheet in PDF
Product Name
GLS2, Polyclonal Antibody
Full Product Name
GLS2 Antibody
Product Gene Name
anti-GLS2 antibody
Product Synonym Names
GLS2; GA; GLS; LGA; hLGA; GA; Glutaminase liver isoform; mitochondrial; L-glutaminase; glutaminase 2 (liver, mitochondrial)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Liquid
Purity/Purification
GLS2 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
Multiple isoforms of GLS2 are known to exist.
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
GLS2 peptide (MBS151918) is used for blocking the activity of GLS2 antibody (MBS150300)
Molecular Weight
Predicted: 36; 55; 62; 66 kDa

Observed: 55; 62 kDa
OMIM
606365
3D Structure
ModBase 3D Structure for Q9UI32
Conjugate
Unconjugated
Immunogen
GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.
Buffer
GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-GLS2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GLS2 antibody
GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Optimal dilutions for each application to be determined by the researcher.

Western Blot (WB) of anti-GLS2 antibody

Western blot analysis of GLS2 in rat liver tissue lysate with GLS2 antibody at (A) 1 and (B) 2 μg/mL.
Rabbit Anti-GLS2 Polyclonal Antibody – Cat# MBS150300

Immunohistochemistry (IHC) of anti-GLS2 antibody

Immunohistochemistry of GLS2 in rat liver tissue with GLS2 antibody at 5 μg/mL.
Rabbit Anti-GLS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150300

Immunofluorescence (IF) of anti-GLS2 antibody

Immunofluorescence of GLS2 in rat liver cells with GLS2 antibody at 20 5 μg/mL.
Rabbit Anti-GLS2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150300

Immunohistochemistry (IHC) of anti-GLS2 antibody

Immunohistochemistry of GLS2 in mouse brain tissue with GLS2 Antibody at 5 μg/mL.
Rabbit Anti-GLS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS150300

Immunofluorescence (IF) of anti-GLS2 antibody

Immunofluorescence of GLS2 in mouse brain tissue with GLS2 Antibody at 20 μg/mL.

Green: GLS2 antibody (6217)
Red: Phylloidin staining
Blue: DAPI staining
Rabbit Anti-GLS2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150300

Other Notes

Small volumes of anti-GLS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GLS2 purchase

MBS150300 is an antibody targeting GLS2. Also known as GLS2 Antibody. Reactive in Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GLS2. It may not necessarily be applicable to this product.

NCBI GI #
20336214
NCBI GeneID
27165
NCBI Accession #
NP_037399
UniProt Primary Accession #
Q9UI32
UniProt Secondary Accession #
Q8IX91; Q9NYY2; Q9UI31; B7Z8Q9
UniProt Related Accession #
Q9UI32
GenBank Nucleotide #
NM_013267.3
NCBI Official Full Name
glutaminase liver isoform, mitochondrial isoform 1
NCBI Official Synonym Full Names
glutaminase 2 (liver, mitochondrial)
NCBI Official Symbol
GLS2
NCBI Official Synonym Symbols
GA; GLS; LGA; hLGA
UniProt Protein Name
Glutaminase liver isoform, mitochondrial
UniProt Synonym Protein Names
L-glutaminase; L-glutamine amidohydrolase
Protein Family
Glucosidase
UniProt Gene Name
GLS2
UniProt Synonym Gene Names
GA; GLS
UniProt Entry Name
GLSL_HUMAN
NCBI Protein Information
glutaminase liver isoform, mitochondrial; glutaminase I; breast cell glutaminase; L-glutamine amidohydrolase; phosphate-activated glutaminase; phosphate-dependent glutaminase

NCBI Summary for GLS2

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
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UniProt Comments for GLS2

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.

Protein type: Mitochondrial; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; EC 3.5.1.2; Hydrolase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; glutaminase activity

Biological Process: amino acid metabolic process; regulation of apoptosis; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process
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Research Articles on GLS2

1. Silencing GLS or overexpressing GLS2 induces growth inhibition in glioma cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GLS2 antibody

 Reference Product  PubMed Publications
 CPS1 antibody  >2 publications with GLS2 and CPS1
 GLUL antibody  >1 publications with GLS2 and GLUL

Pathways associated with anti-GLS2 antibody

 Products by Pathway  Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 D-Glutamine And D-glutamate Metabolism Pathway antibodies  D-Glutamine And D-glutamate Metabolism Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram

Diseases associated with anti-GLS2 antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >4 publications with GLS2 and Liver Diseases
 Brain Diseases Antibodies  >2 publications with GLS2 and Brain Diseases
 Nervous System Diseases Antibodies  >2 publications with GLS2 and Nervous System Diseases
 Lung Neoplasms Antibodies  >2 publications with GLS2 and Lung Neoplasms
 Liver Neoplasms Antibodies  >2 publications with GLS2 and Liver Neoplasms
 Seizures Antibodies  >1 publications with GLS2 and Seizures
 Mental Disorders Antibodies  >1 publications with GLS2 and Mental Disorders
 Kidney Diseases Antibodies  >1 publications with GLS2 and Kidney Diseases
 Drug-Related Side Effects and Adverse Reactions Antibodies  >1 publications with GLS2 and Drug-Related Side Effects and Adverse Reactions
 Carcinoma, Hepatocellular Antibodies  >1 publications with GLS2 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-GLS2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >9 publications with GLS2 and Liver
 Brain Antibodies  >9 publications with GLS2 and Brain
 Kidney Antibodies  >4 publications with GLS2 and Kidney
 Lung Antibodies  >2 publications with GLS2 and Lung
 Thyroid Antibodies  >1 publications with GLS2 and Thyroid
 Skin Antibodies  >1 publications with GLS2 and Skin
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